Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2198815..2199317 Replicon chromosome
Accession NZ_CP012376
Organism Escherichia coli strain GE3

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AKN41_RS10820 Protein ID WP_000767829.1
Coordinates 2198815..2199069 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AKN41_RS10825 Protein ID WP_001259255.1
Coordinates 2199066..2199317 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKN41_RS10790 2193830..2194057 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
AKN41_RS10795 2194071..2195129 - 1059 WP_000492346.1 transport protein YeeE -
AKN41_RS10800 2195308..2196666 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AKN41_RS10805 2196656..2196718 - 63 WP_010723108.1 membrane protein YoeI -
AKN41_RS10810 2196933..2197862 - 930 WP_096096267.1 LysR family transcriptional regulator -
AKN41_RS10815 2197908..2198732 - 825 WP_000754749.1 SDR family oxidoreductase -
AKN41_RS10820 2198815..2199069 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AKN41_RS10825 2199066..2199317 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AKN41_RS10830 2199600..2199650 + 51 WP_001364200.1 his operon leader peptide -
AKN41_RS10835 2199796..2200695 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
AKN41_RS10840 2200701..2202005 + 1305 WP_001369878.1 histidinol dehydrogenase -
AKN41_RS10845 2202002..2203072 + 1071 WP_000108962.1 histidinol-phosphate transaminase -
AKN41_RS10850 2203072..2204139 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T56345 WP_000767829.1 NZ_CP012376:c2199069-2198815 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T56345 NZ_CP012376:c2199069-2198815 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT56345 WP_001259255.1 NZ_CP012376:c2199317-2199066 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT56345 NZ_CP012376:c2199317-2199066 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References