Detailed information of TA system TA056326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2308401..2308978
• Replicon: chromosome
• Accession: NZ_CP012375
• Organism: Microcystis aeruginosa NIES-2481

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2H6BV23
• Locus tag: amyaer_RS11385
• Protein ID: WP_024968549.1
• Coordinates: 2308401..2308679 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: amyaer_RS11390
• Protein ID: WP_046662171.1
• Coordinates: 2308679..2308978 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
amyaer_RS11370	2303718..2305451	-	1734	WP_046662170.1	peptide ABC transporter substrate-binding protein	-
amyaer_RS11375	2306134..2307255	+	1122	WP_149039030.1	DNA cytosine methyltransferase	-
amyaer_RS11380	2307239..2308348	+	1110	WP_045359490.1	hypothetical protein	-
amyaer_RS11385	2308401..2308679	+	279	WP_024968549.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
amyaer_RS11390	2308679..2308978	+	300	WP_046662171.1	HigA family addiction module antidote protein	Antitoxin
amyaer_RS11395	2309170..2310048	-	879	WP_046662172.1	UDP-N-acetylmuramate dehydrogenase	-
amyaer_RS11400	2310168..2311595	-	1428	WP_046662173.1	UDP-N-acetylmuramate--L-alanine ligase	-
amyaer_RS11410	2311851..2312537	-	687	WP_046662175.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10369.75 Da
• Isoelectric Point: 7.3596

>T56326 WP_024968549.1 NZ_CP012375:2308401-2308679 [Microcystis aeruginosa NIES-2481]
MIVSFKHRGLEQFFESGTTRGIQANHAQRIRLILARLSAATSPQDMNLPGLVLHELAGQRKGTWTVRVSGNWRITFTFDG
VDACDVDLEDYH

Nucleotide

• Length: 279 bp

>T56326 NZ_CP012375:2308401-2308679 [Microcystis aeruginosa NIES-2481]
ATGATAGTCAGTTTCAAGCATCGAGGACTAGAGCAGTTCTTTGAGTCGGGAACCACCCGAGGCATTCAGGCAAATCATGC
CCAACGAATTCGGCTGATTCTGGCTCGTCTCAGTGCTGCAACTTCACCGCAAGACATGAACTTACCCGGGTTGGTACTTC
ATGAACTGGCAGGGCAACGTAAAGGAACCTGGACGGTGAGAGTATCTGGAAACTGGCGGATCACTTTCACCTTTGACGGT
GTTGATGCTTGTGATGTCGATCTTGAGGACTACCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10910.65 Da
• Isoelectric Point: 8.0442

>AT56326 WP_046662171.1 NZ_CP012375:2308679-2308978 [Microcystis aeruginosa NIES-2481]
MKMHNPPHPGEVLKELCLEPLNLTVTEAAEALGVSRKTLSAILNGRAGISPEMAIRLGKAFDTSPESWLNQQMQYDLWQA
EQTIGNIKVKRLSVRSAIA

Nucleotide

• Length: 300 bp

>AT56326 NZ_CP012375:2308679-2308978 [Microcystis aeruginosa NIES-2481]
ATGAAAATGCATAATCCACCACATCCTGGTGAAGTTCTTAAAGAACTTTGTCTCGAACCTTTAAATCTGACTGTGACTGA
GGCTGCTGAAGCATTGGGTGTAAGTCGAAAAACCCTATCTGCCATCTTAAATGGTAGGGCAGGTATTAGCCCCGAAATGG
CAATTCGCTTAGGGAAAGCTTTCGATACATCTCCTGAAAGTTGGCTCAATCAGCAAATGCAATACGATCTATGGCAAGCA
GAGCAGACAATTGGCAATATCAAAGTCAAACGGCTATCAGTGCGATCGGCGATCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A2H6BV23