Detailed information of TA system TA056318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 830440..831062
• Replicon: chromosome
• Accession: NZ_CP012371
• Organism: Nitrosospira briensis C-128

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: F822_RS03775
• Protein ID: WP_025040700.1
• Coordinates: 830440..830721 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F822_RS03780
• Protein ID: WP_025040701.1
• Coordinates: 830733..831062 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F822_RS03750	825660..826241	-	582	WP_025040695.1	hypothetical protein	-
F822_RS03755	826505..826915	-	411	WP_082204672.1	hypothetical protein	-
F822_RS03760	827238..827465	-	228	WP_025040697.1	nitrate/nitrite transporter NrtS	-
F822_RS03765	827623..828852	-	1230	WP_082204673.1	ADP-ribosylglycohydrolase family protein	-
F822_RS03770	829091..830305	+	1215	WP_025040699.1	MFS transporter	-
F822_RS03775	830440..830721	+	282	WP_025040700.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F822_RS03780	830733..831062	+	330	WP_025040701.1	HigA family addiction module antidote protein	Antitoxin
F822_RS03785	831646..832350	+	705	WP_025040702.1	ABC transporter ATP-binding protein	-
F822_RS03790	832338..834905	+	2568	WP_036575432.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (12-87)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11072.76 Da
• Isoelectric Point: 9.9053

>T56318 WP_025040700.1 NZ_CP012371:830440-830721 [Nitrosospira briensis C-128]
MILSFKDKETEEFWLTGKSRKLPATLKKRAYARLQALHATEDIKDLLVPPGNQLERLKGERKAEHSIRINKQWRICFIWR
DGDTHDVKIEDYH

Nucleotide

• Length: 282 bp

>T56318 NZ_CP012371:830440-830721 [Nitrosospira briensis C-128]
ATGATACTTAGTTTTAAAGACAAAGAGACAGAAGAGTTTTGGCTTACGGGTAAAAGCCGAAAACTTCCAGCAACTCTTAA
AAAAAGAGCTTATGCCCGCCTTCAGGCACTACACGCGACCGAAGATATAAAGGATCTTCTCGTTCCTCCCGGCAATCAGT
TGGAAAGGCTGAAGGGTGAGCGGAAGGCTGAGCATAGTATCCGTATAAATAAACAATGGCGCATCTGTTTTATCTGGAGA
GATGGCGATACGCATGATGTAAAAATCGAAGACTATCACTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12461.48 Da
• Isoelectric Point: 9.5103

>AT56318 WP_025040701.1 NZ_CP012371:830733-831062 [Nitrosospira briensis C-128]
VTTKDNRRNLISHPGEILKEEFLEPLGISVYALAKAMSVPRTRLNDIVLKRRGISADTALRLARFFDMTPNYWLNLQTHY
DLVMAETEPENTLAKVLTLKDIKKEAIHA

Nucleotide

• Length: 330 bp

>AT56318 NZ_CP012371:830733-831062 [Nitrosospira briensis C-128]
GTGACTACAAAAGACAACCGCAGGAACCTGATTTCTCATCCCGGCGAGATACTCAAAGAGGAGTTTCTAGAGCCGCTTGG
TATTAGCGTATATGCCTTAGCAAAGGCCATGAGTGTTCCCCGTACGCGCCTAAACGACATTGTTTTGAAAAGGCGCGGTA
TTTCTGCCGATACGGCCCTGCGCCTTGCTCGCTTCTTCGACATGACGCCGAATTACTGGCTGAATCTTCAAACGCACTAT
GACCTTGTTATGGCCGAGACTGAGCCTGAAAATACGCTGGCCAAAGTTCTAACCTTGAAAGATATCAAGAAAGAAGCCAT
CCATGCGTAA