Detailed information of TA system TA056205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2905150..2905795
• Replicon: chromosome
• Accession: NZ_CP012266
• Organism: Cronobacter dublinensis subsp. dublinensis LMG 23823

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K8B4F6
• Locus tag: AFK67_RS13330
• Protein ID: WP_007731981.1
• Coordinates: 2905433..2905795 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: K8AR60
• Locus tag: AFK67_RS13325
• Protein ID: WP_007731980.1
• Coordinates: 2905150..2905446 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK67_RS13315	2902808..2903656	-	849	WP_007731976.1	DNA-3-methyladenine glycosylase 2	-
AFK67_RS13320	2903792..2905144	+	1353	WP_038883250.1	molecular chaperone	-
AFK67_RS13325	2905150..2905446	-	297	WP_007731980.1	XRE family transcriptional regulator	Antitoxin
AFK67_RS13330	2905433..2905795	-	363	WP_007731981.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AFK67_RS13335	2906025..2907266	+	1242	WP_053532900.1	MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit	-
AFK67_RS13340	2907266..2910388	+	3123	WP_007723441.1	MdtB/MuxB family multidrug efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-86)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13979.18 Da
• Isoelectric Point: 10.2305

>T56205 WP_007731981.1 NZ_CP012266:c2905795-2905433 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
MTWTVIFTPHFYKWYQKQPEGLQDRIAALLWNLRHSGPLVGRPLVDTIKGSRIPNLKELRVQYGGSPWRVFFAFDPTRQA
VVLCAGNKRGHKDFYKTLITQAETAFFQHLQIMECKNENA

Nucleotide

• Length: 363 bp

>T56205 NZ_CP012266:c2905795-2905433 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
ATGACATGGACGGTGATTTTTACCCCGCATTTTTATAAGTGGTATCAAAAACAGCCAGAAGGATTACAGGATCGTATCGC
CGCGCTGTTATGGAATTTACGCCACAGCGGCCCGTTGGTCGGGCGTCCGCTGGTGGATACGATAAAAGGCTCGCGGATCC
CCAATCTGAAAGAGCTGCGCGTTCAGTATGGCGGTTCGCCGTGGCGGGTATTTTTTGCCTTTGATCCAACCCGTCAGGCT
GTGGTGCTGTGTGCAGGCAACAAGCGCGGTCACAAAGATTTTTATAAAACCTTAATCACGCAGGCGGAGACGGCGTTCTT
CCAGCATCTACAAATAATGGAGTGTAAAAATGAAAACGCTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10922.85 Da
• Isoelectric Point: 5.8852

>AT56205 WP_007731980.1 NZ_CP012266:c2905446-2905150 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
MKTLDNVMAEFTPERQAEILRMADEIALEHGLPRLREERAFSQQQLAALMGVTQPAIAAIEQRGKEIKLLTLKRYVEALG
GKLSLLVELPEGSKIIPV

Nucleotide

• Length: 297 bp

>AT56205 NZ_CP012266:c2905446-2905150 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
ATGAAAACGCTTGATAATGTGATGGCGGAGTTTACGCCAGAGCGGCAGGCTGAGATCCTGCGCATGGCCGATGAAATCGC
CCTTGAACACGGGCTGCCGCGCCTTCGCGAAGAGCGAGCCTTCTCGCAGCAGCAGCTGGCGGCGTTAATGGGCGTCACGC
AGCCCGCTATCGCCGCCATCGAACAGCGCGGAAAGGAGATAAAACTGTTAACGCTTAAACGCTATGTCGAAGCGCTGGGC
GGCAAGCTGTCCCTGCTGGTTGAGCTGCCCGAAGGCAGCAAAATCATTCCTGTTTAA

Structures

Toxin

• AlphaFold DB: K8B4F6

Antitoxin

• AlphaFold DB: K8AR60