Detailed information of TA system TA056193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 171710..172311
• Replicon: chromosome
• Accession: NZ_CP012266
• Organism: Cronobacter dublinensis subsp. dublinensis LMG 23823

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K8AZX2
• Locus tag: AFK67_RS00815
• Protein ID: WP_007711052.1
• Coordinates: 171710..172021 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AFK67_RS00820
• Protein ID: WP_007711054.1
• Coordinates: 172021..172311 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK67_RS00800	168708..169859	+	1152	WP_007711026.1	L-threonine dehydrogenase	-
AFK67_RS00805	169884..170756	-	873	WP_007711046.1	ROK family protein	-
AFK67_RS00810	170913..171515	+	603	WP_007711049.1	LysE family transporter	-
AFK67_RS00815	171710..172021	+	312	WP_007711052.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AFK67_RS00820	172021..172311	+	291	WP_007711054.1	helix-turn-helix domain-containing protein	Antitoxin
AFK67_RS00825	172378..173307	-	930	WP_007711057.1	formate dehydrogenase accessory protein FdhE	-
AFK67_RS00830	173304..173939	-	636	WP_007667684.1	formate dehydrogenase cytochrome b556 subunit	-
AFK67_RS00835	173936..174838	-	903	WP_007711061.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11898.74 Da
• Isoelectric Point: 10.1359

>T56193 WP_007711052.1 NZ_CP012266:171710-172021 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
MLFIETEIFTRAVTALLSDDEYGEFQRFLATQPCSGDVIPETGGLRKARWQANGRGKRGGVRVIYFYRSQTDEIRLLLIY
RKGIKDALSPQEKTVLRMLNARW

Nucleotide

• Length: 312 bp

>T56193 NZ_CP012266:171710-172021 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
ATGTTATTTATTGAAACGGAAATCTTCACCCGGGCCGTGACCGCCCTCCTGAGTGATGACGAATACGGAGAGTTTCAGCG
ATTTCTGGCGACACAACCCTGCTCCGGCGACGTGATCCCCGAGACCGGCGGGCTTCGCAAAGCGCGCTGGCAGGCGAATG
GTCGGGGTAAGCGAGGCGGCGTCAGGGTAATCTATTTCTACCGTTCGCAGACGGATGAAATTCGACTCCTGCTGATTTAT
CGCAAGGGGATAAAAGACGCGCTCTCGCCTCAGGAAAAAACAGTGCTCAGAATGCTCAACGCGAGGTGGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10633.17 Da
• Isoelectric Point: 10.7279

>AT56193 WP_007711054.1 NZ_CP012266:172021-172311 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
MDKALFEQLAASMRQMNEIDEGSREPSRTFTIDALKIKEIRQASGLSQTKFASLIAVSVDTLRNWEQGRRSPTGPARALL
RAIARDPQHVIPALAS

Nucleotide

• Length: 291 bp

>AT56193 NZ_CP012266:172021-172311 [Cronobacter dublinensis subsp. dublinensis LMG 23823]
ATGGACAAGGCGCTTTTTGAACAGTTAGCAGCAAGTATGCGGCAGATGAATGAAATCGATGAAGGCAGCCGCGAGCCTTC
ACGAACTTTCACCATTGATGCGCTGAAAATAAAAGAAATCAGACAGGCATCCGGGCTCTCGCAAACGAAATTCGCCAGCC
TGATTGCCGTGAGCGTTGATACATTACGCAACTGGGAGCAGGGCCGACGCTCTCCTACCGGGCCTGCGCGTGCGCTGCTG
CGAGCCATCGCCCGGGACCCGCAGCACGTGATTCCGGCGCTGGCTTCGTAA

Structures

Toxin

• AlphaFold DB: K8AZX2