Detailed information of TA system TA056172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2853249..2853896
• Replicon: chromosome
• Accession: NZ_CP012257
• Organism: Cronobacter universalis NCTC 9529

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A376EP62
• Locus tag: AFK65_RS13180
• Protein ID: WP_007699659.1
• Coordinates: 2853549..2853896 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AFK65_RS13175
• Protein ID: WP_007699657.1
• Coordinates: 2853249..2853548 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK65_RS13150	2848657..2849223	+	567	WP_007699646.1	DedA family protein	-
AFK65_RS13155	2849220..2849993	-	774	WP_007699648.1	SDR family oxidoreductase	-
AFK65_RS21160	2850166..2850249	+	84	WP_000691708.1	protein YohP	-
AFK65_RS13160	2850286..2851242	-	957	WP_038856749.1	MBL fold metallo-hydrolase	-
AFK65_RS13165	2851318..2852241	+	924	WP_038856748.1	LysR family transcriptional regulator	-
AFK65_RS13170	2852244..2853176	-	933	WP_038856747.1	tRNA dihydrouridine(16) synthase DusC	-
AFK65_RS13175	2853249..2853548	-	300	WP_007699657.1	XRE family transcriptional regulator	Antitoxin
AFK65_RS13180	2853549..2853896	-	348	WP_007699659.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AFK65_RS13185	2854151..2854552	+	402	WP_007699662.1	CidA/LrgA family protein	-
AFK65_RS13190	2854549..2855244	+	696	WP_007699666.1	CidB/LrgB family autolysis modulator	-
AFK65_RS13195	2855374..2856258	+	885	WP_038856746.1	cytidine deaminase	-
AFK65_RS13200	2856383..2857096	+	714	WP_007699681.1	outer membrane permeability protein SanA	-
AFK65_RS13205	2857458..2858468	-	1011	WP_007699685.1	galactose/methyl galactoside ABC transporter permease MglC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (16-88)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13477.75 Da
• Isoelectric Point: 10.2049

>T56172 WP_007699659.1 NZ_CP012257:c2853896-2853549 [Cronobacter universalis NCTC 9529]
MWTIYLTHEFECWLARQPQPLQEDVLAALGLLKIKGPHLGRPYADTLKGSRHAGMKELRVQHAGRPLRAFYAFDPRRYAI
VLCAAEKKGDEKRFYRVMLRVADKLFSHYLNHREV

Nucleotide

• Length: 348 bp

>T56172 NZ_CP012257:c2853896-2853549 [Cronobacter universalis NCTC 9529]
ATGTGGACTATTTATCTCACTCATGAGTTTGAGTGCTGGCTCGCGAGGCAGCCGCAGCCGTTGCAGGAGGATGTGCTGGC
GGCGCTGGGGCTTCTGAAAATCAAAGGCCCGCACCTGGGGCGTCCCTATGCCGATACGCTTAAAGGCTCGCGCCACGCGG
GCATGAAGGAATTACGGGTTCAGCACGCCGGGCGGCCCCTGCGCGCCTTTTACGCTTTTGATCCGCGCCGTTACGCCATT
GTGCTGTGCGCAGCGGAGAAAAAGGGCGATGAAAAGCGGTTTTACCGGGTCATGCTGCGCGTCGCTGACAAACTGTTTAG
CCACTATCTGAACCACCGGGAGGTATAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10947.58 Da
• Isoelectric Point: 5.1423

>AT56172 WP_007699657.1 NZ_CP012257:c2853548-2853249 [Cronobacter universalis NCTC 9529]
MKTFDEVLAAQTPEARARIEEKTAQLLLETHLCALREALSVSQTEMARQMGIAQPSVAAIEQRGNDMKISTLKRYVDALG
GTVRLDVELPDGQRFGFTL

Nucleotide

• Length: 300 bp

>AT56172 NZ_CP012257:c2853548-2853249 [Cronobacter universalis NCTC 9529]
ATGAAGACGTTTGATGAAGTGCTGGCGGCCCAGACCCCGGAGGCCCGCGCGCGCATTGAAGAGAAAACGGCGCAGCTGCT
GCTGGAAACCCACCTCTGTGCGCTGCGCGAGGCCCTCAGCGTGTCGCAAACCGAGATGGCGCGCCAGATGGGCATTGCGC
AGCCGTCGGTCGCGGCTATCGAACAGCGCGGCAACGACATGAAAATCTCCACGCTCAAACGCTATGTCGACGCCCTCGGC
GGCACGGTGCGGCTGGATGTCGAGCTGCCGGACGGCCAGCGTTTCGGTTTTACGCTCTGA

Structures

Toxin

• AlphaFold DB: A0A376EP62