Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2802610..2803256 Replicon chromosome
Accession NZ_CP012257
Organism Cronobacter universalis NCTC 9529

Toxin (Protein)


Gene name higB Uniprot ID A0A376EPA5
Locus tag AFK65_RS12970 Protein ID WP_007699539.1
Coordinates 2802903..2803256 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A376ENR3
Locus tag AFK65_RS12965 Protein ID WP_007699536.1
Coordinates 2802610..2802906 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFK65_RS12955 2800264..2801112 - 849 WP_038856776.1 DNA-3-methyladenine glycosylase 2 -
AFK65_RS12960 2801248..2802600 + 1353 WP_007699533.1 molecular chaperone -
AFK65_RS12965 2802610..2802906 - 297 WP_007699536.1 helix-turn-helix transcriptional regulator Antitoxin
AFK65_RS12970 2802903..2803256 - 354 WP_007699539.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AFK65_RS12975 2803487..2804734 + 1248 WP_038856775.1 MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit -
AFK65_RS12980 2804734..2807856 + 3123 WP_038856774.1 MdtB/MuxB family multidrug efflux RND transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(14-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(34-86)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13756.17 Da        Isoelectric Point: 10.5401

>T56171 WP_007699539.1 NZ_CP012257:c2803256-2802903 [Cronobacter universalis NCTC 9529]
MTWTVIMCPEFATWYEKQDRAVQKRLSAYLMHLSQLGPALGRPLVDTLKTSRYGQLKELRAQCQGKPWRILFAFDPCRRA
ILLCGGDKTGNKRFYSIYIRLAEARFYRHLLALRSER

Download         Length: 354 bp

>T56171 NZ_CP012257:c2803256-2802903 [Cronobacter universalis NCTC 9529]
ATGACATGGACGGTGATCATGTGTCCGGAATTCGCCACATGGTATGAGAAGCAAGACCGTGCGGTGCAAAAACGGCTTTC
AGCGTATCTGATGCATTTAAGTCAGCTCGGGCCTGCACTTGGGCGCCCGCTGGTCGATACGTTAAAAACGTCGCGCTATG
GTCAACTCAAGGAGCTGCGCGCCCAGTGTCAGGGAAAGCCGTGGCGCATCTTGTTCGCCTTTGATCCCTGTCGCCGCGCA
ATTTTACTGTGCGGCGGCGATAAAACCGGGAATAAACGTTTTTATTCGATTTATATCAGGCTGGCGGAAGCGCGTTTTTA
CAGGCACCTGCTGGCGTTAAGGAGTGAGAGATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10803.62 Da        Isoelectric Point: 4.8840

>AT56171 WP_007699536.1 NZ_CP012257:c2802906-2802610 [Cronobacter universalis NCTC 9529]
MKTLDDVLAGYSAEEYAEVLRMADEIALEHGLPRIREARAFSQQQLAEIMGVTQPAIAAIEQRGKEIKLLTLKRYVEALG
GKLSLLIELPEGSKVIPV

Download         Length: 297 bp

>AT56171 NZ_CP012257:c2802906-2802610 [Cronobacter universalis NCTC 9529]
ATGAAAACACTGGATGACGTGCTGGCAGGCTATTCCGCAGAAGAGTACGCCGAAGTATTACGCATGGCTGACGAAATCGC
GCTGGAACACGGCCTGCCGCGCATTCGCGAAGCGCGGGCGTTTTCGCAGCAGCAGCTGGCGGAGATAATGGGCGTAACAC
AGCCCGCTATCGCCGCCATTGAGCAGCGCGGCAAGGAGATCAAACTGCTGACGCTCAAGCGCTATGTCGAAGCGCTGGGC
GGCAAGCTCTCTTTGCTGATAGAGCTGCCCGAAGGCAGCAAAGTGATCCCGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376EPA5

Antitoxin

Source ID Structure
AlphaFold DB A0A376ENR3

References