Detailed information of TA system TA056162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2128737..2129302
• Replicon: chromosome
• Accession: NZ_CP012257
• Organism: Cronobacter universalis NCTC 9529

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A376EGB6
• Locus tag: AFK65_RS09835
• Protein ID: WP_032805395.1
• Coordinates: 2128737..2129015 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: AFK65_RS09840
• Protein ID: WP_038857220.1
• Coordinates: 2129015..2129302 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK65_RS09820	2123797..2125731	+	1935	WP_038857225.1	HAMP domain-containing protein	-
AFK65_RS09825	2125846..2126832	+	987	WP_038857222.1	SDR family oxidoreductase	-
AFK65_RS09830	2126834..2128438	-	1605	WP_007697768.1	alpha-amylase family protein	-
AFK65_RS09835	2128737..2129015	+	279	WP_032805395.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AFK65_RS09840	2129015..2129302	+	288	WP_038857220.1	HigA family addiction module antidote protein	Antitoxin
AFK65_RS09845	2129362..2131476	-	2115	WP_038857219.1	TonB-dependent receptor	-
AFK65_RS09850	2131753..2132811	+	1059	WP_038857217.1	YncE family protein	-
AFK65_RS09855	2132843..2133709	-	867	WP_007697789.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10642.12 Da
• Isoelectric Point: 10.1526

>T56162 WP_032805395.1 NZ_CP012257:2128737-2129015 [Cronobacter universalis NCTC 9529]
MIRSFRHKGLQRYFETGSTAGIHARHARKISLRLAVLNQAIRPADVDLPGFFLHPLTGERQGIWAVTVSGNWRITFEFRD
GDVFIVNYEDYH

Nucleotide

• Length: 279 bp

>T56162 NZ_CP012257:2128737-2129015 [Cronobacter universalis NCTC 9529]
ATGATCAGAAGCTTCAGGCATAAAGGCCTGCAACGCTATTTCGAAACGGGTTCCACCGCAGGCATTCATGCCCGCCATGC
CAGAAAGATTTCTCTGCGGCTGGCGGTGCTGAATCAGGCGATAAGACCCGCTGACGTCGACTTACCGGGCTTTTTCCTGC
ATCCCCTGACGGGGGAGCGTCAAGGTATATGGGCTGTGACCGTCAGCGGAAACTGGCGCATCACGTTTGAGTTTCGGGAT
GGCGATGTCTTCATCGTCAACTATGAGGATTACCACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10400.02 Da
• Isoelectric Point: 8.7494

>AT56162 WP_038857220.1 NZ_CP012257:2129015-2129302 [Cronobacter universalis NCTC 9529]
MATMFNPPHPGKLVQESMEALGLSARALAKALDVAPSTVQRLLVGKSDVSPEMALRLSAVLGSSPHVWLGMQNEYDLFQA
RQSVDFSHLQRLQRA

Nucleotide

• Length: 288 bp

>AT56162 NZ_CP012257:2129015-2129302 [Cronobacter universalis NCTC 9529]
ATGGCAACGATGTTTAACCCACCACACCCAGGCAAGCTGGTGCAGGAGTCAATGGAAGCGCTCGGCCTCAGCGCCCGCGC
GCTGGCCAAAGCGCTGGACGTCGCGCCGTCGACGGTGCAGCGACTGCTGGTCGGCAAATCTGACGTCTCCCCGGAAATGG
CGCTGCGTCTCTCGGCGGTCCTTGGCAGCTCGCCGCATGTCTGGCTGGGCATGCAGAATGAATACGATCTCTTTCAGGCG
CGCCAGTCTGTTGATTTCTCGCATCTGCAACGGTTGCAGAGGGCATAA

Structures

Toxin

• AlphaFold DB: A0A376EGB6