Detailed information of TA system TA056161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1816736..1817406
• Replicon: chromosome
• Accession: NZ_CP012257
• Organism: Cronobacter universalis NCTC 9529

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A376EC11
• Locus tag: AFK65_RS08315
• Protein ID: WP_007696702.1
• Coordinates: 1816736..1817032 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AFK65_RS08320
• Protein ID: WP_007696705.1
• Coordinates: 1817065..1817406 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK65_RS08295	1812345..1813172	-	828	WP_007696687.1	ammonia-dependent NAD(+) synthetase	-
AFK65_RS08300	1813415..1813756	+	342	WP_007696690.1	osmotically-inducible lipoprotein OsmE	-
AFK65_RS08305	1813878..1816133	-	2256	WP_038857366.1	catalase HPII	-
AFK65_RS08310	1816345..1816575	+	231	WP_038857365.1	cell division activator CedA	-
AFK65_RS08315	1816736..1817032	+	297	WP_007696702.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AFK65_RS08320	1817065..1817406	+	342	WP_007696705.1	HigA family addiction module antidote protein	Antitoxin
AFK65_RS08325	1817508..1818899	-	1392	WP_007696707.1	L-cystine transporter	-
AFK65_RS08330	1819035..1819625	-	591	WP_007696709.1	metal-dependent hydrolase	-
AFK65_RS08335	1819739..1820494	-	756	WP_007696711.1	2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD	-
AFK65_RS08340	1820680..1821348	-	669	WP_007696714.1	hexitol phosphatase HxpB	-
AFK65_RS08345	1821507..1822043	+	537	WP_007696717.1	YniB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-69)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11444.03 Da
• Isoelectric Point: 9.3376

>T56161 WP_007696702.1 NZ_CP012257:1816736-1817032 [Cronobacter universalis NCTC 9529]
MQKHIGSFRDAWLAAFFVYSMPHRNIPAEIHNTLARKLDIINAATSYLDLRSPPGNRFEALSGKLQGYSSIRVNHHYRLI
FRWVEGKAEELYLDPHDY

Nucleotide

• Length: 297 bp

>T56161 NZ_CP012257:1816736-1817032 [Cronobacter universalis NCTC 9529]
ATGCAGAAGCATATCGGCAGTTTTCGTGACGCCTGGCTGGCGGCTTTTTTCGTGTATTCGATGCCACACCGCAACATTCC
GGCAGAGATTCATAACACACTGGCGCGCAAACTCGACATCATCAACGCAGCAACCTCATACCTCGATCTCCGCTCGCCGC
CTGGCAACCGGTTTGAGGCGCTGAGCGGTAAATTGCAGGGGTATTCATCGATTCGGGTCAACCATCACTACCGGCTGATT
TTCAGATGGGTGGAAGGCAAAGCGGAAGAGCTCTATTTAGATCCGCATGATTACTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12897.89 Da
• Isoelectric Point: 6.9827

>AT56161 WP_007696705.1 NZ_CP012257:1817065-1817406 [Cronobacter universalis NCTC 9529]
MKQATRKPTTPGEILVYEYLEPLGLKINEVAEMLCVHRNSVSALVNNNRKLTTDMAFRLAKAFGTSVDFWLNLQAAVDLW
EVENDMRIQEAFSRIIPAAEFIARRDTQKKEVA

Nucleotide

• Length: 342 bp

>AT56161 NZ_CP012257:1817065-1817406 [Cronobacter universalis NCTC 9529]
ATGAAACAGGCAACCAGAAAGCCCACGACGCCCGGCGAGATCCTGGTGTACGAATACCTGGAGCCGCTCGGCCTTAAAAT
CAACGAGGTGGCGGAGATGCTTTGCGTCCATCGTAATAGCGTGAGCGCGCTGGTAAACAATAACCGCAAGCTCACTACCG
ATATGGCGTTTCGCCTGGCGAAAGCGTTCGGCACCTCTGTCGACTTCTGGCTTAACCTGCAGGCGGCTGTCGACCTGTGG
GAAGTGGAAAATGATATGCGCATTCAGGAAGCGTTCAGCCGCATCATACCCGCCGCGGAGTTTATCGCCCGGCGCGACAC
GCAGAAGAAAGAAGTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A376EC11