Detailed information of TA system TA056159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 106691..107292
• Replicon: chromosome
• Accession: NZ_CP012257
• Organism: Cronobacter universalis NCTC 9529

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A376DWG1
• Locus tag: AFK65_RS00475
• Protein ID: WP_007704085.1
• Coordinates: 106691..107002 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AFK65_RS00480
• Protein ID: WP_007704087.1
• Coordinates: 107002..107292 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK65_RS00460	103687..104838	+	1152	WP_038858435.1	L-threonine dehydrogenase	-
AFK65_RS00465	104863..105735	-	873	WP_038858432.1	ROK family protein	-
AFK65_RS00470	105892..106491	+	600	WP_007704082.1	LysE family transporter	-
AFK65_RS00475	106691..107002	+	312	WP_007704085.1	hypothetical protein	Toxin
AFK65_RS00480	107002..107292	+	291	WP_007704087.1	helix-turn-helix domain-containing protein	Antitoxin
AFK65_RS00485	107365..108294	-	930	WP_038858423.1	formate dehydrogenase accessory protein FdhE	-
AFK65_RS00490	108291..108926	-	636	WP_004385165.1	formate dehydrogenase cytochrome b556 subunit	-
AFK65_RS00495	108923..109825	-	903	WP_007766109.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12045.00 Da
• Isoelectric Point: 10.2301

>T56159 WP_007704085.1 NZ_CP012257:106691-107002 [Cronobacter universalis NCTC 9529]
MLFIETEIFTRAVTTLLSDAEYQRLQWFLALRPDYGDVIPDTGGLRKARWLSGGRGKRGGVRIIYFYRAAADEIRLLLIY
RKGIKDDLSPHEKSVLRMLNERW

Nucleotide

• Length: 312 bp

>T56159 NZ_CP012257:106691-107002 [Cronobacter universalis NCTC 9529]
ATGCTCTTTATTGAAACGGAAATCTTTACCCGGGCCGTGACAACTCTGCTTAGTGATGCCGAATACCAGCGGTTGCAGTG
GTTTCTGGCCCTGCGGCCTGATTACGGCGACGTGATACCGGATACCGGCGGGCTTCGTAAGGCGCGCTGGCTGTCAGGCG
GCCGGGGAAAACGGGGCGGCGTCAGGATCATCTATTTCTATCGCGCTGCTGCCGATGAAATCCGCCTGCTGCTGATTTAT
CGCAAGGGGATAAAAGACGATCTCTCGCCTCACGAAAAATCTGTGCTCAGAATGTTGAATGAGAGGTGGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10616.12 Da
• Isoelectric Point: 10.9368

>AT56159 WP_007704087.1 NZ_CP012257:107002-107292 [Cronobacter universalis NCTC 9529]
MDNALFEQLTESLRQMNDISEGRREPSRAFVIDALKIKAIRQASGLSQTKFASLISVSVDTLRNWEQGRRSPTGPARALL
RAIANDPQHVLSALAL

Nucleotide

• Length: 291 bp

>AT56159 NZ_CP012257:107002-107292 [Cronobacter universalis NCTC 9529]
ATGGACAACGCGCTGTTTGAGCAGTTAACCGAAAGCCTGCGGCAGATGAATGATATCAGCGAAGGCCGCCGCGAGCCCTC
ACGAGCTTTTGTCATCGACGCGCTGAAAATAAAAGCGATCAGACAGGCATCAGGCCTTTCTCAGACGAAATTCGCCAGCC
TGATTTCGGTAAGCGTGGATACGCTGCGCAACTGGGAGCAGGGAAGACGCTCGCCAACCGGGCCTGCGCGTGCGCTGTTG
CGTGCGATTGCGAACGATCCGCAACATGTGCTCTCTGCGCTTGCGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A376DWG1