Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1175035..1175680 Replicon chromosome
Accession NZ_CP012253
Organism Cronobacter sakazakii strain NCTC 8155

Toxin (Protein)


Gene name higB Uniprot ID A0A6L3QTA3
Locus tag AFK64_RS05535 Protein ID WP_004388208.1
Coordinates 1175035..1175397 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6L3TU83
Locus tag AFK64_RS05540 Protein ID WP_004388209.1
Coordinates 1175384..1175680 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFK64_RS05525 1170435..1173557 - 3123 WP_004388206.1 MdtB/MuxB family multidrug efflux RND transporter permease subunit -
AFK64_RS05530 1173557..1174804 - 1248 WP_004388207.1 MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit -
AFK64_RS05535 1175035..1175397 + 363 WP_004388208.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AFK64_RS05540 1175384..1175680 + 297 WP_004388209.1 XRE family transcriptional regulator Antitoxin
AFK64_RS05545 1175690..1177042 - 1353 WP_004388210.1 molecular chaperone -
AFK64_RS05550 1177178..1178026 + 849 WP_015386509.1 DNA-3-methyladenine glycosylase 2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(34-86)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 14197.07 Da        Isoelectric Point: 8.0154

>T56145 WP_004388208.1 NZ_CP012253:1175035-1175397 [Cronobacter sakazakii]
MAWTVIFTDRFNDWYQQQPEGLQDRIAALLWNLRYSGPLVGRPLVDTVKGSRYPNLKELRVQYGGEPWRVFFAFDAERQA
VVLCAGNKRSQKHFYDALIRQAEEEFFLHVQAMERRNENS

Download         Length: 363 bp

>T56145 NZ_CP012253:1175035-1175397 [Cronobacter sakazakii]
ATGGCATGGACGGTAATTTTTACCGATCGTTTTAACGACTGGTATCAGCAGCAGCCCGAAGGATTACAGGATCGTATCGC
CGCGCTCTTATGGAACTTACGTTATTCCGGCCCGCTTGTCGGACGCCCGCTGGTCGATACGGTGAAAGGTTCGCGTTATC
CCAACCTGAAAGAGCTACGTGTGCAGTATGGCGGTGAGCCCTGGCGCGTCTTTTTTGCGTTTGACGCTGAACGTCAGGCC
GTTGTGCTCTGCGCAGGCAATAAACGAAGCCAGAAACATTTTTATGACGCCTTAATCAGGCAGGCTGAAGAAGAATTCTT
CCTGCATGTGCAAGCGATGGAGAGACGAAATGAAAACTCTTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10837.75 Da        Isoelectric Point: 5.8787

>AT56145 WP_004388209.1 NZ_CP012253:1175384-1175680 [Cronobacter sakazakii]
MKTLDDVMAGFSPERQAEILRMADEIALEHGLPRIREARAFSQQQLAEIMGVTQPAIAAIEQRGKEIKLLTLKRYVEALG
GKLSLLIELPEGSKVIPV

Download         Length: 297 bp

>AT56145 NZ_CP012253:1175384-1175680 [Cronobacter sakazakii]
ATGAAAACTCTTGATGACGTAATGGCGGGCTTTTCCCCGGAACGGCAGGCAGAAATCCTGCGCATGGCAGACGAAATTGC
GCTGGAGCACGGCCTGCCGCGCATTCGCGAAGCGCGGGCGTTTTCGCAGCAGCAGCTGGCGGAGATAATGGGCGTGACGC
AGCCCGCTATCGCCGCCATTGAGCAGCGCGGTAAGGAGATTAAACTGCTGACGCTCAAGCGCTACGTCGAAGCGCTGGGC
GGCAAGCTCTCTTTACTGATAGAACTGCCCGAAGGCAGCAAAGTGATCCCCGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6L3QTA3

Antitoxin

Source ID Structure
AlphaFold DB A0A6L3TU83

References