Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1119413..1120060 Replicon chromosome
Accession NZ_CP012253
Organism Cronobacter sakazakii strain NCTC 8155

Toxin (Protein)


Gene name higB Uniprot ID A0A2S9UA56
Locus tag AFK64_RS05305 Protein ID WP_004387788.1
Coordinates 1119413..1119760 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V5TYW5
Locus tag AFK64_RS05310 Protein ID WP_007776551.1
Coordinates 1119761..1120060 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFK64_RS05275 1114705..1115715 + 1011 WP_004387466.1 galactose/methyl galactoside ABC transporter permease MglC -
AFK64_RS05285 1116215..1116928 - 714 WP_004387784.1 outer membrane permeability protein SanA -
AFK64_RS05290 1117053..1117937 - 885 WP_004387785.1 cytidine deaminase -
AFK64_RS05295 1118067..1118762 - 696 WP_004387786.1 CidB/LrgB family autolysis modulator -
AFK64_RS05300 1118759..1119160 - 402 WP_004387787.1 CidA/LrgA family protein -
AFK64_RS05305 1119413..1119760 + 348 WP_004387788.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AFK64_RS05310 1119761..1120060 + 300 WP_007776551.1 XRE family transcriptional regulator Antitoxin
AFK64_RS05315 1120131..1121063 + 933 WP_004387790.1 tRNA dihydrouridine(16) synthase DusC -
AFK64_RS05320 1121066..1121989 - 924 WP_015386496.1 LysR family transcriptional regulator -
AFK64_RS05325 1122062..1123021 + 960 WP_029464216.1 MBL fold metallo-hydrolase -
AFK64_RS21680 1123058..1123141 - 84 WP_000691708.1 protein YohP -
AFK64_RS05330 1123314..1124087 + 774 WP_004387792.1 SDR family oxidoreductase -
AFK64_RS05335 1124084..1124650 - 567 WP_004387793.1 DedA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-83)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13457.72 Da        Isoelectric Point: 10.2057

>T56144 WP_004387788.1 NZ_CP012253:1119413-1119760 [Cronobacter sakazakii]
MWTIYLTHEFECWLARQPQPLQEDVLAALGLLKIKGPHLGRPYADTLKGSRHAGMKELRVQHAGRPIRAFYAFDPHRYAI
VLCAAQKKGDEKRFYRVMLRVADKLFSHYLNHREV

Download         Length: 348 bp

>T56144 NZ_CP012253:1119413-1119760 [Cronobacter sakazakii]
ATGTGGACTATTTACCTTACTCACGAGTTTGAGTGCTGGCTCGCGAGACAGCCGCAGCCGTTGCAGGAAGATGTGCTGGC
CGCGCTGGGGCTTTTGAAAATCAAAGGTCCGCACCTCGGGCGTCCGTATGCCGATACGCTCAAAGGCTCGCGTCACGCGG
GTATGAAGGAATTACGGGTTCAGCACGCCGGGCGGCCCATACGCGCCTTTTACGCTTTTGATCCGCACCGTTATGCGATT
GTGCTGTGCGCGGCACAGAAAAAGGGGGATGAAAAGCGGTTTTACCGGGTCATGCTGCGCGTCGCTGACAAACTGTTTAG
CCACTATCTGAACCACCGGGAGGTATAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11035.67 Da        Isoelectric Point: 5.1667

>AT56144 WP_007776551.1 NZ_CP012253:1119761-1120060 [Cronobacter sakazakii]
MKTFDEVLAAQTPEARARIEEKTAQLLLETHLYALREALSVSQTEMARQMGIAQPSVAAIEQRGNEMKISTLKRYVEALG
GTVRLDVELPDGQRFGFTL

Download         Length: 300 bp

>AT56144 NZ_CP012253:1119761-1120060 [Cronobacter sakazakii]
ATGAAGACGTTTGATGAAGTGCTGGCGGCGCAGACCCCGGAGGCCCGCGCGCGTATCGAAGAGAAAACGGCGCAGCTGCT
GCTGGAAACCCATCTGTATGCGCTGCGCGAGGCGCTCAGCGTGTCGCAAACCGAGATGGCGCGCCAGATGGGTATCGCCC
AGCCGTCGGTCGCGGCTATCGAACAGCGCGGCAACGAGATGAAAATCTCAACGCTTAAGCGCTATGTCGAAGCCCTGGGC
GGCACGGTGCGACTGGATGTCGAGCTGCCGGACGGCCAACGTTTCGGTTTTACGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S9UA56

Antitoxin

Source ID Structure
AlphaFold DB A0A2S9UA72

References