Detailed information of TA system TA056113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 41693..42209
• Replicon: plasmid unnamed2
• Accession: NZ_CP012189
• Organism: Lacticaseibacillus paracasei strain CAUH35

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: AKG30_RS14220
• Protein ID: WP_050894578.1
• Coordinates: 41928..42209 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: AKG30_RS14215
• Protein ID: WP_016363783.1
• Coordinates: 41693..41935 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKG30_RS16055	37167..37502	+	336	WP_080988372.1	TIR domain-containing protein	-
AKG30_RS14195	37422..38837	-	1416	WP_050893464.1	IS5-like element ISLca2 family transposase	-
AKG30_RS14205	39353..40624	+	1272	WP_050894577.1	hypothetical protein	-
AKG30_RS16430	40728..40949	-	222	Protein_42	IS30 family transposase	-
AKG30_RS14210	41197..41565	+	369	WP_003582572.1	YxeA family protein	-
AKG30_RS14215	41693..41935	+	243	WP_016363783.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AKG30_RS14220	41928..42209	+	282	WP_050894578.1	type II toxin-antitoxin system YafQ family toxin	Toxin
AKG30_RS14225	42362..42733	+	372	Protein_46	recombinase family protein	-
AKG30_RS14230	43127..44806	-	1680	WP_050894579.1	D-lactate dehydrogenase	-
AKG30_RS14235	45066..45749	-	684	WP_050894580.1	IS6 family transposase	-
AKG30_RS14240	45933..46691	-	759	WP_016385700.1	NADPH-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..47712	47712

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-62)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11357.95 Da
• Isoelectric Point: 9.1792

>T56113 WP_050894578.1 NZ_CP012189:41928-42209 [Lacticaseibacillus paracasei]
MNKFIYKPAFERQFKKHYKTILKGGRYKKEDFEAVYHKLLQDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Nucleotide

• Length: 282 bp

>T56113 NZ_CP012189:41928-42209 [Lacticaseibacillus paracasei]
ATGAATAAGTTTATTTATAAACCAGCCTTTGAACGTCAATTTAAAAAACATTACAAGACGATTTTAAAAGGGGGGCGCTA
TAAAAAAGAAGATTTTGAAGCTGTTTATCATAAACTATTACAAGATGAACCGTTAGATCCACATTATAATGATCACGCTT
TGGTTAATCGTAAACCAGAGCGTGATTTACATATCAAGCCGGATTGGTTATTAATTTACAAATATGATGGTGAGTTTGTG
CGCTTTATCGACACGGGTTCGCATTCAGATCTTTTTAATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9217.44 Da
• Isoelectric Point: 5.1548

>AT56113 WP_016363783.1 NZ_CP012189:41693-41935 [Lacticaseibacillus paracasei]
MTSQVHFRMSEKEKKDFEIVLSRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE

Nucleotide

• Length: 243 bp

>AT56113 NZ_CP012189:41693-41935 [Lacticaseibacillus paracasei]
ATGACTAGCCAAGTACATTTCAGAATGAGCGAAAAAGAAAAAAAGGACTTCGAGATTGTCCTAAGTCGTGTGGGATTAAC
TCCCGGAGAAGCCTTTCGTATCTTTGCCAAGAAATCCATTGAAGCCGGAGGAATTCCCTTTGAGGTCTCTCAGCCAAACA
CACGTTTAAAAAAGGCTATTAGAAGTCAAGATTACGTTGAATTTAACGATCCTGAGGAAGGGCTGGATTGGTTAAATGAA
TAA