Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 1160449..1161013 Replicon chromosome
Accession NZ_CP012187
Organism Lacticaseibacillus paracasei strain CAUH35

Toxin (Protein)


Gene name yafQ Uniprot ID K6S2P8
Locus tag AKG30_RS05735 Protein ID WP_003602097.1
Coordinates 1160711..1161013 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag AKG30_RS05730 Protein ID WP_003570051.1
Coordinates 1160449..1160718 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKG30_RS05720 1158562..1159377 + 816 WP_003602094.1 DUF2399 domain-containing protein -
AKG30_RS05725 1159422..1160075 + 654 WP_003602095.1 GNAT family N-acetyltransferase -
AKG30_RS05730 1160449..1160718 + 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
AKG30_RS05735 1160711..1161013 + 303 WP_003602097.1 type II toxin-antitoxin system YafQ family toxin Toxin
AKG30_RS16520 1161352..1161495 + 144 WP_162137653.1 hypothetical protein -
AKG30_RS05740 1161722..1162309 + 588 WP_050894510.1 hypothetical protein -
AKG30_RS05745 1162545..1164722 + 2178 WP_003602103.1 RNA-binding transcriptional accessory protein -
AKG30_RS05750 1165156..1165713 - 558 WP_003602105.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-98)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11442.21 Da        Isoelectric Point: 9.9697

>T56111 WP_003602097.1 NZ_CP012187:1160711-1161013 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGDWLLIYKIE
QQDLILTLVRTGSHHNLLGK

Download         Length: 303 bp

>T56111 NZ_CP012187:1160711-1161013 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCCGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCCT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTACATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTGAG
CAGCAAGATCTTATTTTAACCCTAGTTAGAACCGGATCTCATCATAACCTTCTGGGTAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT56111 WP_003570051.1 NZ_CP012187:1160449-1160718 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT56111 NZ_CP012187:1160449-1160718 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAAGAAACTCGCTTGAATATTCGTGTTGATCCGGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACGATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S2P8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References