Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3584623..3585298 Replicon chromosome
Accession NZ_CP012167
Organism Enterobacter hormaechei subsp. steigerwaltii strain 34998

Toxin (Protein)


Gene name higB Uniprot ID A0A837FDC1
Locus tag LI65_RS17890 Protein ID WP_015571638.1
Coordinates 3584623..3584922 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0PXC9
Locus tag LI65_RS17895 Protein ID WP_015571639.1
Coordinates 3584933..3585298 (+) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI65_RS17880 3581000..3583192 + 2193 WP_039269968.1 type I secretion system permease/ATPase -
LI65_RS17885 3583212..3584372 + 1161 WP_085929677.1 HlyD family efflux transporter periplasmic adaptor subunit -
LI65_RS17890 3584623..3584922 + 300 WP_015571638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LI65_RS17895 3584933..3585298 + 366 WP_015571639.1 HigA family addiction module antidote protein Antitoxin
LI65_RS17900 3585325..3586506 - 1182 WP_017382758.1 PLP-dependent aminotransferase family protein -
LI65_RS17905 3586527..3587414 - 888 WP_015571641.1 LysR family transcriptional regulator -
LI65_RS17910 3587512..3588114 + 603 WP_017382759.1 short chain dehydrogenase -
LI65_RS17915 3588111..3588842 - 732 WP_039269965.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11624.29 Da        Isoelectric Point: 9.6776

>T56087 WP_015571638.1 NZ_CP012167:3584623-3584922 [Enterobacter hormaechei subsp. steigerwaltii]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

>T56087 NZ_CP012167:3584623-3584922 [Enterobacter hormaechei subsp. steigerwaltii]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13705.73 Da        Isoelectric Point: 9.5936

>AT56087 WP_015571639.1 NZ_CP012167:3584933-3585298 [Enterobacter hormaechei subsp. steigerwaltii]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Download         Length: 366 bp

>AT56087 NZ_CP012167:3584933-3585298 [Enterobacter hormaechei subsp. steigerwaltii]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FDC1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0PXC9

References