Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 436137..436755 Replicon chromosome
Accession NZ_CP012140
Organism Shigella flexneri 4c strain 1205

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AD871_RS02355 Protein ID WP_001291435.1
Coordinates 436137..436355 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag AD871_RS02360 Protein ID WP_000344797.1
Coordinates 436381..436755 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD871_RS02325 (432029) 432029..432340 - 312 WP_000409911.1 MGMT family protein -
AD871_RS02335 (432719) 432719..433072 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AD871_RS02340 (433114) 433114..434664 - 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AD871_RS02345 (434828) 434828..435298 - 471 WP_000136192.1 YlaC family protein -
AD871_RS02350 (435414) 435414..435965 - 552 Protein_438 maltose O-acetyltransferase -
AD871_RS02355 (436137) 436137..436355 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AD871_RS02360 (436381) 436381..436755 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
AD871_RS02365 (437301) 437301..440450 - 3150 WP_001132484.1 efflux RND transporter permease AcrB -
AD871_RS02370 (440473) 440473..441666 - 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 430671..431999 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T55999 WP_001291435.1 NZ_CP012140:c436355-436137 [Shigella flexneri 4c]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T55999 NZ_CP012140:c436355-436137 [Shigella flexneri 4c]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT55999 WP_000344797.1 NZ_CP012140:c436755-436381 [Shigella flexneri 4c]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT55999 NZ_CP012140:c436755-436381 [Shigella flexneri 4c]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References