Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 398955..399799 Replicon chromosome
Accession NZ_CP012140
Organism Shigella flexneri 4c strain 1205

Toxin (Protein)


Gene name itaT Uniprot ID -
Locus tag AD871_RS02155 Protein ID WP_014532172.1
Coordinates 398955..399416 (-) Length 154 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A5H0K0
Locus tag AD871_RS02165 Protein ID WP_005053053.1
Coordinates 399488..399799 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD871_RS02135 (394123) 394123..395853 - 1731 WP_000645013.1 hypothetical protein -
AD871_RS02140 (395906) 395906..398059 - 2154 WP_005060811.1 SEL1-like repeat protein -
AD871_RS02150 (398210) 398210..398907 + 698 WP_223368647.1 IS1 family transposase -
AD871_RS02155 (398955) 398955..399416 - 462 WP_014532172.1 GNAT family N-acetyltransferase Toxin
AD871_RS02165 (399488) 399488..399799 - 312 WP_005053053.1 DUF1778 domain-containing protein Antitoxin
AD871_RS02170 (399983) 399983..400873 - 891 WP_000971346.1 heme o synthase -
AD871_RS02175 (400885) 400885..401214 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
AD871_RS02180 (401214) 401214..401828 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
AD871_RS02185 (401818) 401818..403809 - 1992 Protein_406 cytochrome o ubiquinol oxidase subunit I -
AD871_RS02190 (403831) 403831..404328 - 498 Protein_407 COX aromatic rich motif-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 398404..398907 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-133)

Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 154 a.a.        Molecular weight: 16623.52 Da        Isoelectric Point: 9.9538

>T55998 WP_014532172.1 NZ_CP012140:c399416-398955 [Shigella flexneri 4c]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Download         Length: 462 bp

>T55998 NZ_CP012140:c399416-398955 [Shigella flexneri 4c]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11394.07 Da        Isoelectric Point: 10.3388

>AT55998 WP_005053053.1 NZ_CP012140:c399799-399488 [Shigella flexneri 4c]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT55998 NZ_CP012140:c399799-399488 [Shigella flexneri 4c]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A5H0K0

References