Detailed information of TA system TA055973

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1435817..1436042
• Replicon: chromosome
• Accession: NZ_CP012137
• Organism: Shigella flexneri 2a strain 981

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: AD867_RS07730
• Protein ID: WP_000813254.1
• Coordinates: 1435887..1436042 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1435817..1435875 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AD867_RS31255	1431815..1431984	+	170	Protein_1417	hypothetical protein	-
AD867_RS07690	1432130..1432876	+	747	WP_000788996.1	ATP-binding protein	-
AD867_RS07695	1432891..1433313	+	423	WP_001118168.1	DUF977 family protein	-
AD867_RS07700	1433371..1433727	+	357	WP_005048249.1	hypothetical protein	-
AD867_RS07705	1433820..1434038	+	219	WP_000256998.1	DUF4014 family protein	-
AD867_RS07710	1434040..1434405	+	366	WP_001229297.1	HNH endonuclease signature motif containing protein	-
AD867_RS07715	1434402..1435067	+	666	WP_000208062.1	hypothetical protein	-
AD867_RS07720	1435067..1435432	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	1435817..1435875	-	59	-	-	Antitoxin
AD867_RS07730	1435887..1436042	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
AD867_RS07750	1437379..1437978	+	600	WP_000940329.1	DUF1367 family protein	-
AD867_RS07755	1437978..1438268	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
AD867_RS07760	1438265..1438819	+	555	WP_000640143.1	DUF1133 family protein	-
AD867_RS07765	1438972..1439145	+	174	WP_000504450.1	hypothetical protein	-
AD867_RS07770	1439207..1440346	+	1140	WP_000088354.1	IS3 family transposase	-
AD867_RS07775	1440339..1440899	+	561	WP_072075205.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	1422785..1476966	54181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T55973 WP_000813254.1 NZ_CP012137:1435887-1436042 [Shigella flexneri 2a]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T55973 NZ_CP012137:1435887-1436042 [Shigella flexneri 2a]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT55973 NZ_CP012137:c1435875-1435817 [Shigella flexneri 2a]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin