Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 415666..416284 Replicon chromosome
Accession NZ_CP012137
Organism Shigella flexneri 2a strain 981

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AD867_RS02165 Protein ID WP_001291435.1
Coordinates 415666..415884 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag AD867_RS02170 Protein ID WP_000344797.1
Coordinates 415910..416284 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD867_RS02135 (411558) 411558..411869 - 312 WP_000409911.1 MGMT family protein -
AD867_RS02145 (412248) 412248..412601 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AD867_RS02150 (412643) 412643..414193 - 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AD867_RS02155 (414357) 414357..414827 - 471 WP_000136192.1 YlaC family protein -
AD867_RS02160 (414943) 414943..415494 - 552 Protein_397 maltose O-acetyltransferase -
AD867_RS02165 (415666) 415666..415884 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AD867_RS02170 (415910) 415910..416284 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
AD867_RS02175 (416830) 416830..419978 - 3149 Protein_400 efflux RND transporter permease AcrB -
AD867_RS02180 (420001) 420001..421194 - 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 410200..411528 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T55971 WP_001291435.1 NZ_CP012137:c415884-415666 [Shigella flexneri 2a]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T55971 NZ_CP012137:c415884-415666 [Shigella flexneri 2a]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT55971 WP_000344797.1 NZ_CP012137:c416284-415910 [Shigella flexneri 2a]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT55971 NZ_CP012137:c416284-415910 [Shigella flexneri 2a]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References