Detailed information of TA system TA055970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 378485..379329
• Replicon: chromosome
• Accession: NZ_CP012137
• Organism: Shigella flexneri 2a strain 981

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: AD867_RS01965
• Protein ID: WP_014532172.1
• Coordinates: 378485..378946 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A5H0K0
• Locus tag: AD867_RS01975
• Protein ID: WP_005053053.1
• Coordinates: 379018..379329 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AD867_RS01945 (373653)	373653..375383	-	1731	WP_000645013.1	hypothetical protein	-
AD867_RS01950 (375436)	375436..377589	-	2154	WP_005060811.1	SEL1-like repeat protein	-
AD867_RS01960 (377740)	377740..378437	+	698	WP_223368647.1	IS1 family transposase	-
AD867_RS01965 (378485)	378485..378946	-	462	WP_014532172.1	GNAT family N-acetyltransferase	Toxin
AD867_RS01975 (379018)	379018..379329	-	312	WP_005053053.1	DUF1778 domain-containing protein	Antitoxin
AD867_RS01980 (379513)	379513..380403	-	891	WP_000971346.1	heme o synthase	-
AD867_RS01985 (380415)	380415..380744	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AD867_RS01990 (380744)	380744..381358	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AD867_RS01995 (381348)	381348..383339	-	1992	Protein_365	cytochrome o ubiquinol oxidase subunit I	-
AD867_RS02000 (383361)	383361..383858	-	498	Protein_366	COX aromatic rich motif-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T 4168367..4168870 0.004	flank	IS/Tn	-	-	377934..378437	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-133)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16623.52 Da
• Isoelectric Point: 9.9538

>T55970 WP_014532172.1 NZ_CP012137:c378946-378485 [Shigella flexneri 2a]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 462 bp

>T55970 NZ_CP012137:c378946-378485 [Shigella flexneri 2a]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11394.07 Da
• Isoelectric Point: 10.3388

>AT55970 WP_005053053.1 NZ_CP012137:c379329-379018 [Shigella flexneri 2a]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT55970 NZ_CP012137:c379329-379018 [Shigella flexneri 2a]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A5H0K0