Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 298536..299230 Replicon chromosome
Accession NZ_CP012137
Organism Shigella flexneri 2a strain 981

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag AD867_RS01510 Protein ID WP_001263491.1
Coordinates 298832..299230 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag AD867_RS01505 Protein ID WP_000554759.1
Coordinates 298536..298829 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD867_RS01485 (294175) 294175..294672 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
AD867_RS01490 (294889) 294889..296601 - 1713 Protein_277 flagellar biosynthesis protein FlhA -
AD867_RS01495 (296573) 296573..297358 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
AD867_RS01500 (297429) 297429..298484 + 1056 WP_001226172.1 DNA polymerase IV -
AD867_RS01505 (298536) 298536..298829 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
AD867_RS01510 (298832) 298832..299230 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
AD867_RS01515 (299240) 299240..299692 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
AD867_RS01520 (299938) 299938..300981 + 1044 WP_005053205.1 RNA ligase RtcB family protein -
AD867_RS01525 (301043) 301043..301657 + 615 Protein_284 peptide chain release factor H -
AD867_RS01530 (301714) 301714..303171 - 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
AD867_RS01535 (303433) 303433..303891 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T55969 WP_001263491.1 NZ_CP012137:298832-299230 [Shigella flexneri 2a]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T55969 NZ_CP012137:298832-299230 [Shigella flexneri 2a]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT55969 WP_000554759.1 NZ_CP012137:298536-298829 [Shigella flexneri 2a]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT55969 NZ_CP012137:298536-298829 [Shigella flexneri 2a]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References