Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2070403..2070905 Replicon chromosome
Accession NZ_CP012127
Organism Escherichia coli strain DH1Ec169

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ADZ28_RS10390 Protein ID WP_000767829.1
Coordinates 2070403..2070657 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ADZ28_RS10395 Protein ID WP_001259255.1
Coordinates 2070654..2070905 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADZ28_RS10365 2065418..2065645 - 228 WP_000234896.1 sulfurtransferase TusA -
ADZ28_RS10370 2065659..2066717 - 1059 WP_000492339.1 transport protein YeeE -
ADZ28_RS10375 2066896..2068254 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ADZ28_RS25400 2068244..2068306 - 63 WP_010723108.1 membrane protein YoeI -
ADZ28_RS10380 2068521..2069450 - 930 WP_000803351.1 LysR family transcriptional regulator -
ADZ28_RS10385 2069496..2070320 - 825 WP_000754737.1 SDR family oxidoreductase -
ADZ28_RS10390 2070403..2070657 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ADZ28_RS10395 2070654..2070905 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ADZ28_RS23745 2071188..2071238 + 51 WP_001364200.1 his operon leader peptide -
ADZ28_RS10400 2071384..2072283 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ADZ28_RS10405 2072289..2073593 + 1305 WP_000009594.1 histidinol dehydrogenase -
ADZ28_RS10410 2073590..2074660 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
ADZ28_RS10415 2074660..2075727 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T55936 WP_000767829.1 NZ_CP012127:c2070657-2070403 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T55936 NZ_CP012127:c2070657-2070403 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT55936 WP_001259255.1 NZ_CP012127:c2070905-2070654 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT55936 NZ_CP012127:c2070905-2070654 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References