Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2073017..2073519 Replicon chromosome
Accession NZ_CP012126
Organism Escherichia coli strain DH1Ec104

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ADZ27_RS10390 Protein ID WP_000767829.1
Coordinates 2073017..2073271 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ADZ27_RS10395 Protein ID WP_001259255.1
Coordinates 2073268..2073519 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADZ27_RS10365 2068032..2068259 - 228 WP_000234896.1 sulfurtransferase TusA -
ADZ27_RS10370 2068273..2069331 - 1059 WP_000492339.1 transport protein YeeE -
ADZ27_RS10375 2069510..2070868 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ADZ27_RS25395 2070858..2070920 - 63 WP_010723108.1 membrane protein YoeI -
ADZ27_RS10380 2071135..2072064 - 930 WP_000803351.1 LysR family transcriptional regulator -
ADZ27_RS10385 2072110..2072934 - 825 WP_000754737.1 SDR family oxidoreductase -
ADZ27_RS10390 2073017..2073271 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ADZ27_RS10395 2073268..2073519 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ADZ27_RS23730 2073802..2073852 + 51 WP_001364200.1 his operon leader peptide -
ADZ27_RS10400 2073998..2074897 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ADZ27_RS10405 2074903..2076207 + 1305 WP_000009594.1 histidinol dehydrogenase -
ADZ27_RS10410 2076204..2077274 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
ADZ27_RS10415 2077274..2078341 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T55893 WP_000767829.1 NZ_CP012126:c2073271-2073017 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T55893 NZ_CP012126:c2073271-2073017 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT55893 WP_001259255.1 NZ_CP012126:c2073519-2073268 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT55893 NZ_CP012126:c2073519-2073268 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References