Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2073016..2073518 Replicon chromosome
Accession NZ_CP012125
Organism Escherichia coli strain DH1Ec095

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ADS71_RS10390 Protein ID WP_000767829.1
Coordinates 2073016..2073270 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ADS71_RS10395 Protein ID WP_001259255.1
Coordinates 2073267..2073518 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADS71_RS10365 2068031..2068258 - 228 WP_000234896.1 sulfurtransferase TusA -
ADS71_RS10370 2068272..2069330 - 1059 WP_000492339.1 transport protein YeeE -
ADS71_RS10375 2069509..2070867 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ADS71_RS25400 2070857..2070919 - 63 WP_010723108.1 membrane protein YoeI -
ADS71_RS10380 2071134..2072063 - 930 WP_000803351.1 LysR family transcriptional regulator -
ADS71_RS10385 2072109..2072933 - 825 WP_000754737.1 SDR family oxidoreductase -
ADS71_RS10390 2073016..2073270 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ADS71_RS10395 2073267..2073518 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ADS71_RS23750 2073801..2073851 + 51 WP_001364200.1 his operon leader peptide -
ADS71_RS10400 2073997..2074896 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ADS71_RS10405 2074902..2076206 + 1305 WP_000009594.1 histidinol dehydrogenase -
ADS71_RS10410 2076203..2077273 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
ADS71_RS10415 2077273..2078340 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T55850 WP_000767829.1 NZ_CP012125:c2073270-2073016 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T55850 NZ_CP012125:c2073270-2073016 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT55850 WP_001259255.1 NZ_CP012125:c2073518-2073267 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT55850 NZ_CP012125:c2073518-2073267 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References