Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3512558..3513228 Replicon chromosome
Accession NZ_CP012095
Organism Mycobacterium tuberculosis variant bovis strain 1595

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag Mb1595_RS16535 Protein ID WP_003899954.1
Coordinates 3512558..3512902 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag Mb1595_RS16540 Protein ID WP_003899955.1
Coordinates 3512899..3513228 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Mb1595_RS16505 3507631..3508491 + 861 WP_003416628.1 alpha/beta hydrolase -
Mb1595_RS16510 3508466..3508981 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
Mb1595_RS16515 3509221..3509514 + 294 WP_003416635.1 hypothetical protein -
Mb1595_RS16520 3509802..3511091 + 1290 WP_003416640.1 ATP-binding protein -
Mb1595_RS16525 3511438..3511872 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
Mb1595_RS16530 3511875..3512327 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
Mb1595_RS16535 3512558..3512902 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
Mb1595_RS16540 3512899..3513228 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
Mb1595_RS16545 3513766..3514113 + 348 WP_003899956.1 DUF2384 domain-containing protein -
Mb1595_RS16550 3514110..3514730 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
Mb1595_RS16555 3514890..3516155 - 1266 WP_003909837.1 hypothetical protein -
Mb1595_RS16560 3516323..3516532 + 210 WP_003416778.1 hypothetical protein -
Mb1595_RS22105 3516567..3516752 + 186 WP_003908177.1 hypothetical protein -
Mb1595_RS16570 3516779..3517813 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T55747 WP_003899954.1 NZ_CP012095:3512558-3512902 [Mycobacterium tuberculosis variant bovis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T55747 NZ_CP012095:3512558-3512902 [Mycobacterium tuberculosis variant bovis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT55747 WP_003899955.1 NZ_CP012095:3512899-3513228 [Mycobacterium tuberculosis variant bovis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT55747 NZ_CP012095:3512899-3513228 [Mycobacterium tuberculosis variant bovis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References