Detailed information of TA system TA055747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3512558..3513228
• Replicon: chromosome
• Accession: NZ_CP012095
• Organism: Mycobacterium tuberculosis variant bovis strain 1595

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: Mb1595_RS16535
• Protein ID: WP_003899954.1
• Coordinates: 3512558..3512902 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: Mb1595_RS16540
• Protein ID: WP_003899955.1
• Coordinates: 3512899..3513228 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Mb1595_RS16505	3507631..3508491	+	861	WP_003416628.1	alpha/beta hydrolase	-
Mb1595_RS16510	3508466..3508981	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
Mb1595_RS16515	3509221..3509514	+	294	WP_003416635.1	hypothetical protein	-
Mb1595_RS16520	3509802..3511091	+	1290	WP_003416640.1	ATP-binding protein	-
Mb1595_RS16525	3511438..3511872	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
Mb1595_RS16530	3511875..3512327	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
Mb1595_RS16535	3512558..3512902	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Mb1595_RS16540	3512899..3513228	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
Mb1595_RS16545	3513766..3514113	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
Mb1595_RS16550	3514110..3514730	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
Mb1595_RS16555	3514890..3516155	-	1266	WP_003909837.1	hypothetical protein	-
Mb1595_RS16560	3516323..3516532	+	210	WP_003416778.1	hypothetical protein	-
Mb1595_RS22105	3516567..3516752	+	186	WP_003908177.1	hypothetical protein	-
Mb1595_RS16570	3516779..3517813	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T55747 WP_003899954.1 NZ_CP012095:3512558-3512902 [Mycobacterium tuberculosis variant bovis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T55747 NZ_CP012095:3512558-3512902 [Mycobacterium tuberculosis variant bovis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT55747 WP_003899955.1 NZ_CP012095:3512899-3513228 [Mycobacterium tuberculosis variant bovis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT55747 NZ_CP012095:3512899-3513228 [Mycobacterium tuberculosis variant bovis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6