Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2198318..2199186 Replicon chromosome
Accession NZ_CP012095
Organism Mycobacterium tuberculosis variant bovis strain 1595

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag Mb1595_RS10230 Protein ID WP_010886136.1
Coordinates 2198318..2198695 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag Mb1595_RS10235 Protein ID WP_003409886.1
Coordinates 2198737..2199186 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Mb1595_RS10180 2194107..2194559 - 453 WP_010950636.1 lipoprotein -
Mb1595_RS10185 2194623..2195024 + 402 WP_003409869.1 hypothetical protein -
Mb1595_RS10190 2195017..2195199 - 183 WP_003409870.1 hypothetical protein -
Mb1595_RS10195 2195313..2195663 - 351 WP_003409871.1 hypothetical protein -
Mb1595_RS10200 2195674..2196576 - 903 WP_003409874.1 hypothetical protein -
Mb1595_RS10205 2196597..2196788 - 192 WP_003409876.1 hypothetical protein -
Mb1595_RS10210 2196789..2197085 - 297 WP_003409877.1 hypothetical protein -
Mb1595_RS10215 2197325..2197540 + 216 WP_003409878.1 antitoxin -
Mb1595_RS10220 2197537..2197848 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
Mb1595_RS22060 2197822..2198343 - 522 WP_010950637.1 hypothetical protein -
Mb1595_RS10230 2198318..2198695 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
Mb1595_RS10235 2198737..2199186 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
Mb1595_RS10240 2199183..2199728 + 546 WP_003409891.1 SecB-like chaperone -
Mb1595_RS10245 2199617..2200231 - 615 WP_003901296.1 hypothetical protein -
Mb1595_RS10250 2200280..2200576 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
Mb1595_RS10255 2200573..2200824 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
Mb1595_RS10260 2200811..2201305 + 495 WP_003899099.1 hypothetical protein -
Mb1595_RS10265 2201465..2201872 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
Mb1595_RS10270 2201876..2202148 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
Mb1595_RS10275 2202181..2203401 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T55724 WP_010886136.1 NZ_CP012095:2198318-2198695 [Mycobacterium tuberculosis variant bovis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T55724 NZ_CP012095:2198318-2198695 [Mycobacterium tuberculosis variant bovis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT55724 WP_003409886.1 NZ_CP012095:2198737-2199186 [Mycobacterium tuberculosis variant bovis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT55724 NZ_CP012095:2198737-2199186 [Mycobacterium tuberculosis variant bovis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References