Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2037949..2038868 Replicon chromosome
Accession NZ_CP012090
Organism Mycobacterium tuberculosis W-148

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag TBPG_RS21195 Protein ID WP_003900449.1
Coordinates 2038263..2038868 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag TBPG_RS09405 Protein ID WP_003410124.1
Coordinates 2037949..2038254 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBPG_RS09365 2033212..2033412 + 201 Protein_1847 hypothetical protein -
TBPG_RS09380 2034768..2035145 + 378 Protein_1849 hypothetical protein -
TBPG_RS09385 2035142..2036182 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
TBPG_RS09390 2036424..2037143 + 720 WP_003410108.1 DUF433 domain-containing protein -
TBPG_RS09395 2037133..2037549 + 417 WP_003410114.1 hypothetical protein -
TBPG_RS09400 2037565..2037864 - 300 WP_003410120.1 hypothetical protein -
TBPG_RS09405 2037949..2038254 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
TBPG_RS21195 2038263..2038868 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
TBPG_RS09415 2038893..2039252 - 360 WP_003410131.1 hypothetical protein -
TBPG_RS09420 2039412..2040089 - 678 WP_003905725.1 hypothetical protein -
TBPG_RS09425 2040041..2040277 - 237 WP_003410133.1 hypothetical protein -
TBPG_RS09430 2040484..2041380 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T55660 WP_003900449.1 NZ_CP012090:c2038868-2038263 [Mycobacterium tuberculosis W-148]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T55660 NZ_CP012090:c2038868-2038263 [Mycobacterium tuberculosis W-148]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAGGGGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT55660 WP_003410124.1 NZ_CP012090:c2038254-2037949 [Mycobacterium tuberculosis W-148]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT55660 NZ_CP012090:c2038254-2037949 [Mycobacterium tuberculosis W-148]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCGTTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References