Detailed information of TA system TA055592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 604694..605289
• Replicon: chromosome
• Accession: NZ_CP012066
• Organism: Pseudomonas aeruginosa strain F9676

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: ADJ52_RS29215
• Protein ID: WP_003113526.1
• Coordinates: 604694..604972 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ADJ52_RS02705
• Protein ID: WP_003113527.1
• Coordinates: 604984..605289 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ADJ52_RS02690	600120..601358	+	1239	WP_003113524.1	dipeptidase	-
ADJ52_RS02695	601420..602067	+	648	WP_003095021.1	carbonate dehydratase	-
ADJ52_RS02700	602137..604365	-	2229	WP_023086667.1	TonB-dependent receptor	-
ADJ52_RS29215	604694..604972	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ADJ52_RS02705	604984..605289	+	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
ADJ52_RS30245	605717..606214	+	498	WP_126644794.1	ankyrin repeat domain-containing protein	-
ADJ52_RS29220	606623..608830	+	2208	WP_079381482.1	DEAD/DEAH box helicase	-
ADJ52_RS29225	608827..609501	+	675	WP_079381483.1	DUF2290 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T55592 WP_003113526.1 NZ_CP012066:604694-604972 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T55592 NZ_CP012066:604694-604972 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT55592 WP_003113527.1 NZ_CP012066:604984-605289 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT55592 NZ_CP012066:604984-605289 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3