Detailed information of TA system TA055414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 69995..70653
• Replicon: plasmid pAB04-2
• Accession: NZ_CP012008
• Organism: Acinetobacter baumannii strain Ab04-mff

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ACX61_RS20205
• Protein ID: WP_000312250.1
• Coordinates: 69995..70354 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ACX61_RS20210
• Protein ID: WP_001096429.1
• Coordinates: 70354..70653 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACX61_RS20175	65515..65829	-	315	WP_000708714.1	hypothetical protein	-
ACX61_RS20180	66885..67643	-	759	WP_001053127.1	hypothetical protein	-
ACX61_RS20185	67710..68093	-	384	WP_000654348.1	hypothetical protein	-
ACX61_RS20190	68232..68930	-	699	WP_000873188.1	hypothetical protein	-
ACX61_RS20195	68997..69179	-	183	WP_000373385.1	hypothetical protein	-
ACX61_RS20200	69228..69794	-	567	WP_000710385.1	hypothetical protein	-
ACX61_RS20205	69995..70354	+	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACX61_RS20210	70354..70653	+	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
ACX61_RS20215	71191..71475	-	285	WP_049592568.1	hypothetical protein	-
ACX61_RS20220	71540..72565	+	1026	WP_079454613.1	IS30-like element ISAba125 family transposase	-
ACX61_RS20225	72867..73421	-	555	WP_000790084.1	hypothetical protein	-
ACX61_RS20230	73441..73659	-	219	WP_001043201.1	hypothetical protein	-
ACX61_RS20235	73699..74328	-	630	WP_000701003.1	hypothetical protein	-
ACX61_RS20240	74394..75419	+	1026	WP_010326927.1	IS30 family transposase	-
ACX61_RS20245	75435..75614	-	180	WP_000387630.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-23	-	1..87569	87569

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T55414 WP_000312250.1 NZ_CP012008:69995-70354 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T55414 NZ_CP012008:69995-70354 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT55414 WP_001096429.1 NZ_CP012008:70354-70653 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT55414 NZ_CP012008:70354-70653 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA