Detailed information of TA system TA055386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 230239..230814
• Replicon: chromosome
• Accession: NZ_CP011994
• Organism: Ectothiorhodospira sp. BSL-9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A166ZWK2
• Locus tag: ECTOBSL9_RS01115
• Protein ID: WP_063463512.1
• Coordinates: 230536..230814 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: ECTOBSL9_RS16270
• Protein ID: WP_082829664.1
• Coordinates: 230239..230526 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECTOBSL9_RS01090	225791..226822	-	1032	Protein_206	N-6 DNA methylase	-
ECTOBSL9_RS01095	226855..228492	-	1638	WP_063463344.1	IS66 family transposase	-
ECTOBSL9_RS01100	228552..228917	-	366	WP_063463345.1	IS66 family insertion sequence element accessory protein TnpB	-
ECTOBSL9_RS01105	228914..229297	-	384	WP_063463510.1	helix-turn-helix domain containing protein	-
ECTOBSL9_RS01110	229389..229855	-	467	Protein_210	type I restriction-modification system subunit M N-terminal domain-containing protein	-
ECTOBSL9_RS16265	230001..230132	+	132	WP_082829663.1	maturase	-
ECTOBSL9_RS16270	230239..230526	-	288	WP_082829664.1	HigA family addiction module antidote protein	Antitoxin
ECTOBSL9_RS01115	230536..230814	-	279	WP_063463512.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECTOBSL9_RS01120	230871..231557	+	687	WP_082829665.1	Rpn family recombination-promoting nuclease/putative transposase	-
ECTOBSL9_RS01125	231686..232069	+	384	WP_063463348.1	helix-turn-helix domain containing protein	-
ECTOBSL9_RS01130	232066..232431	+	366	WP_063463347.1	IS66 family insertion sequence element accessory protein TnpB	-
ECTOBSL9_RS01135	232491..234128	+	1638	WP_063463514.1	IS66 family transposase	-
ECTOBSL9_RS16785	234195..234308	-	114	Protein_218	transposase	-
ECTOBSL9_RS16280	234347..234697	-	351	WP_082829667.1	putative addiction module antidote protein	-
ECTOBSL9_RS01145	234706..234993	-	288	WP_063463516.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ECTOBSL9_RS16285	235063..235344	-	282	WP_082829668.1	DUF2442 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-86)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10348.67 Da
• Isoelectric Point: 8.6420

>T55386 WP_063463512.1 NZ_CP011994:c230814-230536 [Ectothiorhodospira sp. BSL-9]
MIRSFEHKGLAKFFKSGSTAGIQAAHARRLRLILGRLNAASDTKDMDLPGLRLHELSGDRTGIWSVTVGGNWRVTFRFED
GDAEIVNYEDYH

Nucleotide

• Length: 279 bp

>T55386 NZ_CP011994:c230814-230536 [Ectothiorhodospira sp. BSL-9]
ATGATTCGAAGCTTCGAACACAAGGGCTTGGCCAAATTCTTCAAGTCCGGCAGCACAGCAGGGATTCAGGCCGCTCACGC
AAGGAGGCTTCGACTCATTCTCGGTAGATTAAATGCGGCGTCGGATACCAAGGATATGGACTTGCCAGGCCTTCGGTTGC
ACGAACTCTCGGGCGATCGCACCGGAATCTGGTCAGTGACAGTGGGTGGTAACTGGCGAGTGACGTTCCGGTTCGAGGAC
GGAGATGCCGAGATAGTGAATTACGAAGATTACCACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10415.07 Da
• Isoelectric Point: 7.3233

>AT55386 WP_082829664.1 NZ_CP011994:c230526-230239 [Ectothiorhodospira sp. BSL-9]
MLMHNPPHPGEVLRELCLEPLGLTVTAAAEGLGVSRKTLSAVLNGKAGISPEMAIRLSIAFNTSAESWLNQQCQYELWHA
EQHRKELNVKKLVAA

Nucleotide

• Length: 288 bp

>AT55386 NZ_CP011994:c230526-230239 [Ectothiorhodospira sp. BSL-9]
ATGTTGATGCACAATCCTCCTCACCCCGGCGAAGTATTGAGAGAGCTTTGCCTTGAGCCCCTGGGCCTGACTGTTACAGC
GGCTGCAGAGGGTCTGGGCGTTAGCCGTAAAACACTGAGTGCCGTGTTGAACGGTAAGGCGGGTATTAGTCCTGAAATGG
CTATCCGACTTTCTATCGCTTTTAATACCTCGGCAGAAAGCTGGCTGAATCAGCAATGCCAATATGAACTCTGGCATGCT
GAACAACATCGCAAAGAGCTCAACGTGAAGAAGCTTGTTGCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A166ZWK2