Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3285219..3285837 Replicon chromosome
Accession NZ_CP011971
Organism Steroidobacter denitrificans strain DSM 18526

Toxin (Protein)


Gene name higB Uniprot ID A0A127FD75
Locus tag ACG33_RS14675 Protein ID WP_066922308.1
Coordinates 3285219..3285548 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A127FD47
Locus tag ACG33_RS14680 Protein ID WP_066922310.1
Coordinates 3285541..3285837 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACG33_RS14645 3280244..3280612 + 369 WP_066922297.1 GIY-YIG nuclease family protein -
ACG33_RS14650 3280584..3281528 - 945 WP_083537034.1 TIGR03619 family F420-dependent LLM class oxidoreductase -
ACG33_RS15905 3281525..3281716 - 192 WP_083537036.1 DUF2726 domain-containing protein -
ACG33_RS16280 3281742..3281960 - 219 WP_157071817.1 DUF2726 domain-containing protein -
ACG33_RS14655 3281982..3283508 - 1527 WP_066922301.1 Fic family protein -
ACG33_RS14660 3283604..3283954 - 351 WP_210399104.1 putative DNA binding domain-containing protein -
ACG33_RS14665 3284054..3284317 + 264 WP_066922305.1 type II toxin-antitoxin system ParD family antitoxin -
ACG33_RS14670 3284317..3284640 + 324 WP_066922306.1 type II toxin-antitoxin system RelE/ParE family toxin -
ACG33_RS15915 3284895..3284999 - 105 Protein_2897 transposase domain-containing protein -
ACG33_RS16620 3285002..3285148 + 147 WP_210399105.1 hypothetical protein -
ACG33_RS14675 3285219..3285548 + 330 WP_066922308.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ACG33_RS14680 3285541..3285837 + 297 WP_066922310.1 helix-turn-helix domain-containing protein Antitoxin
ACG33_RS14685 3285943..3286137 - 195 Protein_2901 antirestriction protein ArdA -
ACG33_RS14690 3286092..3286898 + 807 WP_210399106.1 integrase core domain-containing protein -
ACG33_RS14700 3287173..3287706 + 534 WP_066922312.1 cytochrome c5 family protein -
ACG33_RS14705 3287801..3289292 - 1492 Protein_2904 YifB family Mg chelatase-like AAA ATPase -
ACG33_RS14710 3289359..3289646 - 288 WP_066923542.1 accessory factor UbiK family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(17-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(34-86)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12210.30 Da        Isoelectric Point: 10.5567

>T55300 WP_066922308.1 NZ_CP011971:3285219-3285548 [Steroidobacter denitrificans]
MAWTISFFSSKVEQETLTLPKGILASFLRVVELIEEFGPTIGRPHTAPLGGGLFEIRAKGREGISRSVFCTLKRQEIVVL
ITVIKKGNTIPARHMDTARKRMKEVQDND

Download         Length: 330 bp

>T55300 NZ_CP011971:3285219-3285548 [Steroidobacter denitrificans]
ATGGCATGGACGATCAGTTTCTTCAGTTCCAAGGTCGAGCAAGAGACGTTGACTTTGCCGAAAGGAATATTGGCGTCGTT
CCTTCGTGTCGTGGAGCTGATCGAGGAATTCGGCCCGACGATTGGCAGGCCGCATACCGCGCCCCTCGGCGGCGGATTAT
TCGAGATTCGCGCCAAGGGTCGCGAAGGCATTTCCCGCTCTGTCTTCTGCACGCTCAAGAGGCAGGAGATTGTGGTTTTG
ATAACGGTGATCAAGAAGGGAAACACGATCCCGGCCCGCCATATGGATACGGCACGCAAACGCATGAAGGAGGTACAGGA
CAATGACTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11276.08 Da        Isoelectric Point: 11.1213

>AT55300 WP_066922310.1 NZ_CP011971:3285541-3285837 [Steroidobacter denitrificans]
MTRPTFDQFRKKALRKPGVKAEYEALATAFEMKRRMIALRRAAGLTQEQMAEKLGTRKSNISRLESVNSDVSPRLSTLEN
YARVLGYQLVVEFEPRAR

Download         Length: 297 bp

>AT55300 NZ_CP011971:3285541-3285837 [Steroidobacter denitrificans]
ATGACTAGACCGACATTCGACCAGTTTCGCAAGAAGGCGCTCAGGAAGCCCGGCGTGAAGGCCGAGTACGAGGCGCTCGC
CACGGCATTCGAGATGAAGCGGCGCATGATCGCGCTCCGCCGGGCGGCAGGACTGACGCAAGAACAGATGGCGGAAAAAC
TCGGGACCCGGAAGAGCAATATCTCCCGGCTTGAGAGCGTGAATTCGGACGTGTCGCCCCGCCTCTCGACGCTGGAGAAT
TACGCCCGCGTTCTCGGGTATCAGCTCGTGGTCGAGTTCGAGCCGCGCGCCCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A127FD75

Antitoxin

Source ID Structure
AlphaFold DB A0A127FD47

References