Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 561794..562410 Replicon chromosome
Accession NZ_CP011971
Organism Steroidobacter denitrificans strain DSM 18526

Toxin (Protein)


Gene name higB Uniprot ID A0A127F685
Locus tag ACG33_RS02390 Protein ID WP_066922714.1
Coordinates 561794..562111 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A127F6G1
Locus tag ACG33_RS02395 Protein ID WP_066918388.1
Coordinates 562111..562410 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACG33_RS02365 556944..558176 + 1233 WP_066918382.1 ATP-binding protein -
ACG33_RS02370 558322..560028 + 1707 WP_066918020.1 IS1634 family transposase -
ACG33_RS02380 560580..560870 - 291 WP_066918386.1 XRE family transcriptional regulator -
ACG33_RS02385 560867..561214 - 348 WP_210399147.1 type II toxin-antitoxin system RelE/ParE family toxin -
ACG33_RS02390 561794..562111 + 318 WP_066922714.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ACG33_RS02395 562111..562410 + 300 WP_066918388.1 helix-turn-helix domain-containing protein Antitoxin
ACG33_RS02400 562440..562733 + 294 WP_066918390.1 hypothetical protein -
ACG33_RS02410 563201..563923 - 723 WP_066918392.1 YebC/PmpR family DNA-binding transcriptional regulator -
ACG33_RS02415 564008..564403 - 396 WP_066918394.1 DUF1840 domain-containing protein -
ACG33_RS02420 564517..565611 - 1095 WP_066918396.1 HDOD domain-containing protein -
ACG33_RS02425 566027..566875 + 849 WP_066918398.1 MBL fold metallo-hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(35-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12025.96 Da        Isoelectric Point: 9.7145

>T55291 WP_066922714.1 NZ_CP011971:561794-562111 [Steroidobacter denitrificans]
VIFLETPVFTRQIQALVDDDQYRELQARLMAKPEAGDLIPRSGGLRKIRMGLAGRGKRGGARVIYYWVKPRSQIYMLLAY
AKNEQEDLTADQLKVLKDLVQEEFG

Download         Length: 318 bp

>T55291 NZ_CP011971:561794-562111 [Steroidobacter denitrificans]
GTGATCTTCCTGGAAACACCTGTCTTCACGCGCCAGATTCAGGCGCTGGTTGACGATGACCAATACCGGGAACTGCAAGC
CCGGCTCATGGCCAAGCCCGAGGCAGGCGACCTGATCCCACGCAGCGGGGGATTGCGGAAGATCAGGATGGGTCTGGCAG
GCCGCGGCAAACGCGGCGGTGCCAGGGTCATCTACTACTGGGTAAAGCCCAGATCACAGATTTACATGCTGCTCGCCTAC
GCCAAGAACGAGCAGGAAGACCTGACTGCCGACCAGCTCAAGGTCTTGAAGGACTTGGTACAAGAGGAGTTCGGCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11073.88 Da        Isoelectric Point: 11.7491

>AT55291 WP_066918388.1 NZ_CP011971:562111-562410 [Steroidobacter denitrificans]
MDPKLFAELTKSIKQAGAIARGERKPSRRFEFTPSRIQAVREKTELSQAQFARLLGVSIKTLQNWEQARRAPTGPAKALL
RIMEKEPSAALRALRSEAA

Download         Length: 300 bp

>AT55291 NZ_CP011971:562111-562410 [Steroidobacter denitrificans]
ATGGACCCGAAACTGTTTGCAGAGCTGACCAAAAGCATCAAGCAAGCTGGCGCCATCGCGCGGGGCGAGCGCAAGCCGTC
GCGTCGCTTTGAGTTCACGCCGTCCAGGATTCAGGCTGTGCGGGAGAAGACTGAACTTTCGCAGGCTCAGTTCGCCCGGC
TGCTCGGCGTCAGCATCAAGACGCTGCAGAACTGGGAGCAGGCCCGACGAGCTCCGACCGGACCGGCCAAGGCTCTGCTT
CGGATTATGGAAAAGGAGCCTTCTGCCGCGCTGCGCGCCCTGAGATCAGAAGCGGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A127F685

Antitoxin

Source ID Structure
AlphaFold DB A0A127F6G1

References