Detailed information of TA system TA055269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4737623..4738212
• Replicon: chromosome
• Accession: NZ_CP011960
• Organism: Xanthomonas oryzae pv. oryzicola strain L8

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: ACU14_RS20475
• Protein ID: WP_012443754.1
• Coordinates: 4737623..4737904 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ACU14_RS20480
• Protein ID: WP_011260746.1
• Coordinates: 4737922..4738212 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACU14_RS20470	4735267..4737528	+	2262	WP_048483857.1	exodeoxyribonuclease V subunit alpha	-
ACU14_RS20475	4737623..4737904	+	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACU14_RS20480	4737922..4738212	+	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
ACU14_RS20490	4739165..4740559	+	1395	WP_024712138.1	nucleoside hydrolase	-
ACU14_RS20495	4741011..4741343	-	333	WP_011260743.1	hypothetical protein	-
ACU14_RS20505	4741679..4741903	+	225	WP_044751358.1	hypothetical protein	-
ACU14_RS20510	4742041..4742475	+	435	WP_082350439.1	PAAR domain-containing protein	-
ACU14_RS20515	4742478..4742954	+	477	WP_048483859.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11098.89 Da
• Isoelectric Point: 10.5318

>T55269 WP_012443754.1 NZ_CP011960:4737623-4737904 [Xanthomonas oryzae pv. oryzicola]
MIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T55269 NZ_CP011960:4737623-4737904 [Xanthomonas oryzae pv. oryzicola]
ATGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCGCATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT55269 WP_011260746.1 NZ_CP011960:4737922-4738212 [Xanthomonas oryzae pv. oryzicola]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT55269 NZ_CP011960:4737922-4738212 [Xanthomonas oryzae pv. oryzicola]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAACGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGTTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5