Detailed information of TA system TA055189

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 13560..13986
• Replicon: plasmid pPSUO2
• Accession: NZ_CP011916
• Organism: Escherichia coli strain PSUO2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ACJ74_RS25255
• Protein ID: WP_001312861.1
• Coordinates: 13560..13718 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	13762..13986 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACJ74_RS25215	8609..8836	-	228	WP_001254385.1	conjugal transfer relaxosome protein TraY	-
ACJ74_RS25220	8930..9616	-	687	WP_000332493.1	PAS domain-containing protein	-
ACJ74_RS25225	9807..10190	-	384	WP_000124979.1	relaxosome protein TraM	-
ACJ74_RS25230	10467..11114	+	648	WP_000615259.1	transglycosylase SLT domain-containing protein	-
ACJ74_RS25235	11411..12232	-	822	WP_001234469.1	DUF945 domain-containing protein	-
ACJ74_RS25240	12352..12639	-	288	WP_000107535.1	hypothetical protein	-
ACJ74_RS26365	12664..12870	-	207	WP_000547971.1	hypothetical protein	-
ACJ74_RS25255	13560..13718	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	13762..13986	-	225	NuclAT_0	-	Antitoxin
-	13762..13986	-	225	NuclAT_0	-	Antitoxin
-	13762..13986	-	225	NuclAT_0	-	Antitoxin
-	13762..13986	-	225	NuclAT_0	-	Antitoxin
ACJ74_RS26215	13798..13986	+	189	WP_001299721.1	hypothetical protein	-
ACJ74_RS25260	13998..14717	-	720	WP_000116348.1	plasmid SOS inhibition protein A	-
ACJ74_RS25265	14714..15148	-	435	WP_000845873.1	conjugation system SOS inhibitor PsiB	-
ACJ74_RS25270	15203..17161	-	1959	WP_000117210.1	ParB/RepB/Spo0J family partition protein	-
ACJ74_RS25275	17226..17459	-	234	WP_000006030.1	DUF905 family protein	-
ACJ74_RS25280	17521..18060	-	540	WP_000290812.1	single-stranded DNA-binding protein	-
ACJ74_RS26370	18086..18292	-	207	WP_000547971.1	hypothetical protein	-
ACJ74_RS25300	18533..18760	-	228	WP_071594011.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN	1..110961	110961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T55189 WP_001312861.1 NZ_CP011916:c13718-13560 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T55189 NZ_CP011916:c13718-13560 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT55189 NZ_CP011916:c13986-13762 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin