Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4605816..4606510 Replicon chromosome
Accession NZ_CP011915
Organism Escherichia coli strain PSUO2

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag ACJ74_RS23580 Protein ID WP_001263501.1
Coordinates 4605816..4606214 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag ACJ74_RS23585 Protein ID WP_000554755.1
Coordinates 4606217..4606510 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACJ74_RS23555 4601181..4601639 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
ACJ74_RS23560 4601900..4603357 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
ACJ74_RS23565 4603414..4604028 - 615 WP_000602123.1 peptide chain release factor H -
ACJ74_RS23570 4604025..4605164 - 1140 WP_000521578.1 RNA ligase RtcB family protein -
ACJ74_RS23575 4605354..4605806 - 453 WP_001059897.1 GNAT family N-acetyltransferase -
ACJ74_RS23580 4605816..4606214 - 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
ACJ74_RS23585 4606217..4606510 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
ACJ74_RS23590 4606562..4607617 - 1056 WP_001226170.1 DNA polymerase IV -
ACJ74_RS23595 4607688..4608611 - 924 WP_001232551.1 putative lateral flagellar export/assembly protein LafU -
ACJ74_RS23600 4608614..4609477 - 864 WP_001169519.1 flagellar motor stator protein MotA -
ACJ74_RS23605 4609490..4610209 - 720 WP_000938737.1 FliA/WhiG family RNA polymerase sigma factor -
ACJ74_RS23610 4610229..4610696 - 468 WP_000725264.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T55185 WP_001263501.1 NZ_CP011915:c4606214-4605816 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T55185 NZ_CP011915:c4606214-4605816 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT55185 WP_000554755.1 NZ_CP011915:c4606510-4606217 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT55185 NZ_CP011915:c4606510-4606217 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References