Detailed information of TA system TA055166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 645113..645715
• Replicon: chromosome
• Accession: NZ_CP011915
• Organism: Escherichia coli strain PSUO2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A366YL64
• Locus tag: ACJ74_RS03245
• Protein ID: WP_001519058.1
• Coordinates: 645404..645715 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ACJ74_RS03240
• Protein ID: WP_000356397.1
• Coordinates: 645113..645403 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACJ74_RS03205	641186..642088	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
ACJ74_RS03210	642085..642720	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
ACJ74_RS03215	642717..643646	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
ACJ74_RS03220	643862..644080	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
ACJ74_RS03230	644476..644754	-	279	WP_001296612.1	hypothetical protein	-
ACJ74_RS03240	645113..645403	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ACJ74_RS03245	645404..645715	-	312	WP_001519058.1	hypothetical protein	Toxin
ACJ74_RS03250	645944..646852	+	909	WP_001331553.1	alpha/beta hydrolase	-
ACJ74_RS03255	646916..647857	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ACJ74_RS03260	647902..648339	-	438	WP_000560990.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ACJ74_RS03265	648336..649208	-	873	WP_000920762.1	virulence factor BrkB family protein	-
ACJ74_RS03270	649202..649801	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12202.25 Da
• Isoelectric Point: 10.1861

>T55166 WP_001519058.1 NZ_CP011915:c645715-645404 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYKIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T55166 NZ_CP011915:c645715-645404 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATAAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT55166 WP_000356397.1 NZ_CP011915:c645403-645113 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT55166 NZ_CP011915:c645403-645113 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A366YL64