Detailed information of TA system TA055140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3506445..3507175
• Replicon: chromosome
• Accession: NZ_CP011863
• Organism: Enterobacter asburiae strain ATCC 35953

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A376FEI5
• Locus tag: ACJ69_RS16960
• Protein ID: WP_029741423.1
• Coordinates: 3506861..3507175 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ACJ69_RS16955
• Protein ID: WP_167347079.1
• Coordinates: 3506445..3506864 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACJ69_RS16935	3501917..3503422	-	1506	WP_059347367.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
ACJ69_RS16940	3503419..3504012	-	594	WP_029741420.1	transcriptional regulator UhpA	-
ACJ69_RS16945	3504113..3504400	-	288	WP_010426528.1	acetolactate synthase small subunit	-
ACJ69_RS16950	3504404..3506092	-	1689	WP_059347368.1	acetolactate synthase large subunit	-
ACJ69_RS25340	3506199..3506297	-	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
ACJ69_RS16955	3506445..3506864	-	420	WP_167347079.1	helix-turn-helix domain-containing protein	Antitoxin
ACJ69_RS16960	3506861..3507175	-	315	WP_029741423.1	type II toxin-antitoxin system HigB family toxin	Toxin
ACJ69_RS24420	3507662..3507751	+	90	WP_023309830.1	type I toxin-antitoxin system toxin TisB	-
ACJ69_RS16970	3507816..3508262	+	447	WP_059347369.1	GNAT family N-acetyltransferase	-
ACJ69_RS16975	3508325..3509158	+	834	WP_059347370.1	DMT family transporter	-
ACJ69_RS16980	3509334..3510518	+	1185	WP_029741426.1	multidrug efflux MFS transporter EmrD	-
ACJ69_RS16985	3510421..3511509	-	1089	WP_059347371.1	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12361.46 Da
• Isoelectric Point: 10.2219

>T55140 WP_029741423.1 NZ_CP011863:c3507175-3506861 [Enterobacter asburiae]
MHLISMKAILDAVTQFPQHKDELLFLGRTIEKSHCPTPAALKKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T55140 NZ_CP011863:c3507175-3506861 [Enterobacter asburiae]
ATGCATTTAATCTCTATGAAAGCTATTTTGGATGCGGTAACCCAGTTTCCGCAGCACAAGGATGAACTCCTTTTTTTGGG
TCGCACCATCGAAAAGAGCCATTGCCCAACACCTGCTGCTCTGAAAAAACTGTTTCCGACGCTGGATAACTTTAAGTATC
TGGATAAGCATTATGTTATTGATATTGCAAACAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15487.99 Da
• Isoelectric Point: 4.5348

>AT55140 WP_167347079.1 NZ_CP011863:c3506864-3506445 [Enterobacter asburiae]
MMIVADAMKATHALVAAVPLLGEQPSEKDYEDALELVEYLLMNEPDSPLLDIVCARISRYEANRPDIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFEDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT55140 NZ_CP011863:c3506864-3506445 [Enterobacter asburiae]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATGAGGATGCGCTAGAGCTGGTTGAGTATCTTCTTATGAACGAGCCCGATAGCCCCTTGTTGGATATTGTGT
GCGCCAGGATTAGCCGTTACGAAGCCAATCGGCCAGATATTGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGT
ATTGCGGTTTTAAGAACCTTGATGGATCAGTACAACCTGACGATATCGGATTTTGAGGATGAAATTGGCAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGCGAGGTTTGGGGTATCAC
CTGCGCTGTTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A376FEI5