Detailed information of TA system TA055128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5795172..5795767
• Replicon: chromosome
• Accession: NZ_CP011857
• Organism: Pseudomonas aeruginosa strain ATCC 27853

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: ACG06_RS32880
• Protein ID: WP_003117425.1
• Coordinates: 5795489..5795767 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ACG06_RS27025
• Protein ID: WP_003099268.1
• Coordinates: 5795172..5795477 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACG06_RS27005	5790625..5790915	-	291	WP_023083237.1	DUF5447 family protein	-
ACG06_RS27015	5791127..5791399	-	273	WP_003115921.1	hypothetical protein	-
ACG06_RS27020	5791509..5791775	+	267	WP_016852153.1	hypothetical protein	-
ACG06_RS32865	5791898..5792743	+	846	WP_196645791.1	helix-turn-helix domain-containing protein	-
ACG06_RS32870	5792718..5794256	+	1539	WP_023082696.1	GNAT family N-acetyltransferase	-
ACG06_RS32875	5794268..5794795	-	528	WP_071535723.1	AAA family ATPase	-
ACG06_RS27025	5795172..5795477	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
ACG06_RS32880	5795489..5795767	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACG06_RS27030	5796096..5798324	+	2229	WP_003099265.1	TonB-dependent receptor	-
ACG06_RS27035	5798394..5799041	-	648	WP_003095021.1	carbonate dehydratase	-
ACG06_RS27040	5799103..5800341	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T55128 WP_003117425.1 NZ_CP011857:c5795767-5795489 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T55128 NZ_CP011857:c5795767-5795489 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT55128 WP_003099268.1 NZ_CP011857:c5795477-5795172 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT55128 NZ_CP011857:c5795477-5795172 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5