Detailed information of TA system TA055120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1438064..1438629
• Replicon: chromosome
• Accession: NZ_CP011854
• Organism: Pseudomonas stutzeri strain SLG510A3-8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0H3YSF5
• Locus tag: AB691_RS06895
• Protein ID: WP_048328779.1
• Coordinates: 1438351..1438629 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AB691_RS06890
• Protein ID: WP_048328778.1
• Coordinates: 1438064..1438351 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB691_RS06860	1433909..1434283	-	375	WP_196304876.1	hypothetical protein	-
AB691_RS06865	1434296..1434685	-	390	WP_048328774.1	hypothetical protein	-
AB691_RS06870	1434678..1434887	-	210	WP_175415085.1	hypothetical protein	-
AB691_RS22115	1434925..1435086	-	162	WP_196304889.1	helix-turn-helix domain-containing protein	-
AB691_RS06880	1435353..1435628	+	276	WP_014851980.1	hypothetical protein	-
AB691_RS21960	1435752..1436474	-	723	WP_155416512.1	hypothetical protein	-
AB691_RS21965	1436509..1437120	-	612	WP_155416513.1	hypothetical protein	-
AB691_RS21970	1437107..1437790	-	684	WP_155416514.1	hypothetical protein	-
AB691_RS06885	1437860..1438054	-	195	WP_048328777.1	hypothetical protein	-
AB691_RS06890	1438064..1438351	-	288	WP_048328778.1	HigA family addiction module antidote protein	Antitoxin
AB691_RS06895	1438351..1438629	-	279	WP_048328779.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AB691_RS06900	1438892..1440370	-	1479	WP_048328780.1	MFS transporter	-
AB691_RS06905	1440470..1441015	+	546	WP_013982287.1	TetR/AcrR family transcriptional regulator	-
AB691_RS06910	1441019..1441897	-	879	WP_048328781.1	helix-turn-helix domain-containing protein	-
AB691_RS06915	1442060..1443244	+	1185	WP_048328782.1	4-hydroxybenzoate 3-monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1431284..1438825	7541

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10685.30 Da
• Isoelectric Point: 9.5915

>T55120 WP_048328779.1 NZ_CP011854:c1438629-1438351 [Pseudomonas stutzeri]
MIKTFQHKGLRKLFETGSTAGVQPAHAKRLRMQLAALDTAMTIDDMDIPGFRLHPLKGEMRGRWSIMVNGNWRLTFEFRD
GNAYVLDYEDYH

Nucleotide

• Length: 279 bp

>T55120 NZ_CP011854:c1438629-1438351 [Pseudomonas stutzeri]
ATGATCAAAACGTTCCAGCACAAAGGGCTTCGCAAGCTCTTCGAAACGGGCAGCACGGCCGGCGTTCAGCCAGCCCATGC
CAAGCGGCTACGGATGCAACTGGCAGCCCTGGATACGGCCATGACGATAGACGACATGGATATCCCCGGCTTCCGGCTGC
ACCCGCTCAAGGGCGAAATGCGTGGGCGCTGGTCGATCATGGTGAACGGCAACTGGCGGCTGACGTTCGAGTTTCGCGAC
GGAAACGCTTATGTCCTGGACTATGAGGATTACCACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10495.06 Da
• Isoelectric Point: 6.8784

>AT55120 WP_048328778.1 NZ_CP011854:c1438351-1438064 [Pseudomonas stutzeri]
MSMHNPPHPGEFITDIYLEPNGISGRELAEKLGVAPSTLSRVLKGTSRVTPEMALRLSVALGRSPESWLAMQDTYDLWVA
RQHVDLQHVGKLAFA

Nucleotide

• Length: 288 bp

>AT55120 NZ_CP011854:c1438351-1438064 [Pseudomonas stutzeri]
ATGAGCATGCACAACCCTCCCCATCCTGGTGAGTTCATCACCGATATCTACCTGGAGCCGAATGGCATCAGCGGGCGTGA
GCTGGCGGAAAAGCTAGGCGTTGCCCCGTCCACCCTAAGCCGCGTTTTGAAGGGCACTAGTCGCGTTACGCCGGAAATGG
CCCTGCGTCTGTCCGTAGCGCTTGGCCGCTCGCCTGAGAGCTGGCTGGCCATGCAGGATACCTACGATCTGTGGGTAGCT
CGTCAGCATGTGGACTTGCAGCATGTTGGCAAGCTGGCTTTTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3YSF5