Detailed information of TA system TA055057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4105828..4106440
• Replicon: chromosome
• Accession: NZ_CP011801
• Organism: Nitrospira moscoviensis strain NSP M-1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: NITMOv2_RS20095
• Protein ID: WP_083448245.1
• Coordinates: 4106171..4106440 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NITMOv2_RS20090
• Protein ID: WP_053381552.1
• Coordinates: 4105828..4106157 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NITMOv2_RS23075	4101684..4102043	-	360	WP_187299454.1	hypothetical protein	-
NITMOv2_RS20070	4102124..4103212	+	1089	WP_053381549.1	transposase	-
NITMOv2_RS20075	4103223..4103576	-	354	WP_083448244.1	helix-turn-helix domain-containing protein	-
NITMOv2_RS20080	4103573..4103911	-	339	WP_053381550.1	type II toxin-antitoxin system RelE/ParE family toxin	-
NITMOv2_RS20085	4104720..4105724	+	1005	WP_053381551.1	Fic family protein	-
NITMOv2_RS20090	4105828..4106157	-	330	WP_053381552.1	HigA family addiction module antidote protein	Antitoxin
NITMOv2_RS20095	4106171..4106440	-	270	WP_083448245.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NITMOv2_RS23660	4106436..4106627	-	192	WP_145976449.1	aldose 1-epimerase	-
NITMOv2_RS20100	4106540..4107388	-	849	WP_053381554.1	hypothetical protein	-
NITMOv2_RS20105	4107658..4109520	-	1863	WP_083448247.1	polysaccharide biosynthesis protein	-
NITMOv2_RS20110	4109611..4110228	-	618	WP_053381556.1	YdcF family protein	-
NITMOv2_RS20115	4110237..4111382	-	1146	WP_053381557.1	DegT/DnrJ/EryC1/StrS family aminotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4090399..4110228	19829

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (8-77)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-71)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10219.61 Da
• Isoelectric Point: 9.2527

>T55057 WP_083448245.1 NZ_CP011801:c4106440-4106171 [Nitrospira moscoviensis]
ISGRDRRTRLFFAGETIREFSGFKKVAERKLVMLDSASDLKDLGSPGNRLEKLKGNRAGQHSIRINDQWRICFVWMTDGP
HDVEITDDL

Nucleotide

• Length: 270 bp

>T55057 NZ_CP011801:c4106440-4106171 [Nitrospira moscoviensis]
ATTAGCGGGCGGGACAGGAGAACGAGACTGTTCTTTGCGGGAGAGACGATCAGAGAATTCTCGGGCTTCAAGAAAGTTGC
TGAGCGGAAACTCGTGATGCTCGACAGCGCATCGGATCTGAAAGACCTCGGGTCACCGGGTAACCGGCTTGAGAAGTTGA
AGGGGAATCGAGCCGGGCAGCACAGTATCCGAATTAACGATCAATGGCGGATCTGTTTCGTCTGGATGACAGACGGGCCC
CACGATGTCGAGATTACCGATGACCTCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12077.06 Da
• Isoelectric Point: 9.8829

>AT55057 WP_053381552.1 NZ_CP011801:c4106157-4105828 [Nitrospira moscoviensis]
MMKNGMRPVHPGEILLEEFMKPSAPPLNANTLAKALDVPANRITAIIKGQRGITGDTAVRLATFFNTTAEFWMNLQKTYE
LRLAEQALPGRVRKHIEECREALASASSL

Nucleotide

• Length: 330 bp

>AT55057 NZ_CP011801:c4106157-4105828 [Nitrospira moscoviensis]
ATGATGAAGAACGGCATGCGTCCGGTTCACCCTGGGGAAATTCTGTTGGAAGAGTTTATGAAGCCGTCAGCTCCTCCGCT
TAACGCCAATACACTCGCCAAGGCCCTTGATGTGCCGGCGAATCGGATCACGGCGATTATCAAAGGCCAGCGCGGCATTA
CGGGCGATACGGCAGTGCGGCTGGCTACGTTCTTCAATACGACGGCTGAATTCTGGATGAATCTGCAAAAGACGTATGAG
TTGCGTCTGGCCGAACAAGCCCTGCCGGGCAGGGTGAGGAAGCATATTGAGGAGTGCAGGGAGGCCCTTGCATCGGCGTC
AAGCTTATGA