Detailed information of TA system TA054810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1546540..1547215
• Replicon: chromosome
• Accession: NZ_CP011662
• Organism: Enterobacter hormaechei strain CAV1176

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AB284_RS08745
• Protein ID: WP_032619177.1
• Coordinates: 1546916..1547215 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0PXC9
• Locus tag: AB284_RS08740
• Protein ID: WP_015571639.1
• Coordinates: 1546540..1546905 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB284_RS08720	1542996..1543727	+	732	WP_017382760.1	methyltransferase domain-containing protein	-
AB284_RS08725	1543724..1544326	-	603	WP_017382759.1	short chain dehydrogenase	-
AB284_RS08730	1544424..1545311	+	888	WP_032619173.1	LysR family transcriptional regulator	-
AB284_RS08735	1545332..1546513	+	1182	WP_032619175.1	PLP-dependent aminotransferase family protein	-
AB284_RS08740	1546540..1546905	-	366	WP_015571639.1	HigA family addiction module antidote protein	Antitoxin
AB284_RS08745	1546916..1547215	-	300	WP_032619177.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AB284_RS08750	1547466..1548626	-	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
AB284_RS08755	1548646..1550301	-	1656	Protein_1458	ATP-binding cassette domain-containing protein	-
AB284_RS08760	1550334..1551302	+	969	WP_080288382.1	IS5-like element IS903B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1545980..1583671	37691
	-	flank	IS/Tn	-	-	1550379..1551302	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11564.19 Da
• Isoelectric Point: 9.6776

>T54810 WP_032619177.1 NZ_CP011662:c1547215-1546916 [Enterobacter hormaechei]
MINHSRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T54810 NZ_CP011662:c1547215-1546916 [Enterobacter hormaechei]
ATGATTAATCATTCTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13705.73 Da
• Isoelectric Point: 9.5936

>AT54810 WP_015571639.1 NZ_CP011662:c1546905-1546540 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT54810 NZ_CP011662:c1546905-1546540 [Enterobacter hormaechei]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PXC9