Detailed information of TA system TA054781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3112244..3112919
• Replicon: chromosome
• Accession: NZ_CP011650
• Organism: Enterobacter hormaechei strain CAV1669

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AB285_RS15400
• Protein ID: WP_032619177.1
• Coordinates: 3112244..3112543 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0PXC9
• Locus tag: AB285_RS15405
• Protein ID: WP_015571639.1
• Coordinates: 3112554..3112919 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB285_RS15385	3108157..3109125	-	969	WP_080288382.1	IS5-like element IS903B family transposase	-
AB285_RS15390	3109158..3110813	+	1656	Protein_2900	ATP-binding cassette domain-containing protein	-
AB285_RS15395	3110833..3111993	+	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
AB285_RS15400	3112244..3112543	+	300	WP_032619177.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AB285_RS15405	3112554..3112919	+	366	WP_015571639.1	HigA family addiction module antidote protein	Antitoxin
AB285_RS15410	3112946..3114127	-	1182	WP_032619175.1	PLP-dependent aminotransferase family protein	-
AB285_RS15415	3114148..3115035	-	888	WP_032619173.1	LysR family transcriptional regulator	-
AB285_RS15420	3115133..3115735	+	603	WP_017382759.1	short chain dehydrogenase	-
AB285_RS15425	3115732..3116463	-	732	WP_017382760.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3075788..3113479	37691
	-	flank	IS/Tn	-	-	3108157..3109080	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11564.19 Da
• Isoelectric Point: 9.6776

>T54781 WP_032619177.1 NZ_CP011650:3112244-3112543 [Enterobacter hormaechei]
MINHSRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T54781 NZ_CP011650:3112244-3112543 [Enterobacter hormaechei]
ATGATTAATCATTCTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13705.73 Da
• Isoelectric Point: 9.5936

>AT54781 WP_015571639.1 NZ_CP011650:3112554-3112919 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT54781 NZ_CP011650:3112554-3112919 [Enterobacter hormaechei]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PXC9