Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 68570..69297 Replicon plasmid pCAV1043-97
Accession NZ_CP011590
Organism Enterobacter asburiae strain CAV1043

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag AB190_RS01395 Protein ID WP_000558568.1
Coordinates 68570..68881 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7U3M0L1
Locus tag AB190_RS01400 Protein ID WP_072199090.1
Coordinates 68878..69297 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB190_RS01380 65042..66787 + 1746 WP_004187005.1 ATP-grasp domain-containing protein -
AB190_RS24810 66951..67022 - 72 Protein_71 cellulose synthase -
AB190_RS01385 67069..68037 - 969 WP_074132802.1 IS5 family transposase -
AB190_RS01390 68039..68359 - 321 Protein_73 gluconate permease -
AB190_RS01395 68570..68881 + 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
AB190_RS01400 68878..69297 + 420 WP_072199090.1 helix-turn-helix domain-containing protein Antitoxin
AB190_RS25870 69334..69543 - 210 WP_020806042.1 hypothetical protein -
AB190_RS01410 70118..71122 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
AB190_RS01415 71297..71722 - 426 WP_023316432.1 glutaredoxin-dependent arsenate reductase -
AB190_RS01420 71735..73024 - 1290 WP_032409266.1 arsenic transporter -
AB190_RS01425 73069..73365 - 297 WP_047721846.1 metalloregulator ArsR/SmtB family transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ugd 1..96842 96842
- inside IScluster/Tn - - 67069..74469 7400


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T54636 WP_000558568.1 NZ_CP011590:68570-68881 [Enterobacter asburiae]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T54636 NZ_CP011590:68570-68881 [Enterobacter asburiae]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15592.75 Da        Isoelectric Point: 4.4075

>AT54636 WP_072199090.1 NZ_CP011590:68878-69297 [Enterobacter asburiae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIEALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT54636 NZ_CP011590:68878-69297 [Enterobacter asburiae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGAAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U3M0L1

References