Detailed information of TA system TA054631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4454162..4454892
• Replicon: chromosome
• Accession: NZ_CP011584
• Organism: Enterobacter hormaechei strain CAV1668

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: ABY64_RS21910
• Protein ID: WP_023302825.1
• Coordinates: 4454162..4454476 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ABY64_RS21915
• Protein ID: WP_023302826.1
• Coordinates: 4454473..4454892 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABY64_RS21885	4449181..4450380	-	1200	WP_014603550.1	site-specific integrase	-
ABY64_RS21890	4450819..4452003	-	1185	WP_015570118.1	multidrug efflux MFS transporter EmrD	-
ABY64_RS21895	4452179..4453012	-	834	WP_032620535.1	DMT family transporter	-
ABY64_RS21900	4453075..4453521	-	447	WP_003861064.1	GNAT family N-acetyltransferase	-
ABY64_RS24905	4453586..4453675	-	90	WP_017383683.1	type I toxin-antitoxin system toxin TisB	-
ABY64_RS21910	4454162..4454476	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
ABY64_RS21915	4454473..4454892	+	420	WP_023302826.1	helix-turn-helix domain-containing protein	Antitoxin
ABY64_RS25470	4455039..4455137	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
ABY64_RS21920	4455244..4456932	+	1689	WP_023302827.1	acetolactate synthase large subunit	-
ABY64_RS21925	4456936..4457223	+	288	WP_015570113.1	acetolactate synthase small subunit	-
ABY64_RS21930	4457349..4457942	+	594	WP_003861054.1	transcriptional regulator UhpA	-
ABY64_RS21935	4457939..4459444	+	1506	WP_017694010.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4410686..4461719	51033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T54631 WP_023302825.1 NZ_CP011584:4454162-4454476 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T54631 NZ_CP011584:4454162-4454476 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15380.96 Da
• Isoelectric Point: 5.2068

>AT54631 WP_023302826.1 NZ_CP011584:4454473-4454892 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANGPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT54631 NZ_CP011584:4454473-4454892 [Enterobacter hormaechei]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGTCCTTTGCTGGATATTGTGT
GCGCCAGAATTAGCCATTACGAAGCTAATGGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTAAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACCCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CTGCGTTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A837FIL2