Detailed information of TA system TA054628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3139715..3140390
• Replicon: chromosome
• Accession: NZ_CP011584
• Organism: Enterobacter hormaechei strain CAV1668

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ABY64_RS15565
• Protein ID: WP_032619177.1
• Coordinates: 3139715..3140014 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0PXC9
• Locus tag: ABY64_RS15570
• Protein ID: WP_015571639.1
• Coordinates: 3140025..3140390 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABY64_RS15550	3135628..3136596	-	969	WP_080288382.1	IS5-like element IS903B family transposase	-
ABY64_RS15555	3136629..3138284	+	1656	Protein_2924	ATP-binding cassette domain-containing protein	-
ABY64_RS15560	3138304..3139464	+	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
ABY64_RS15565	3139715..3140014	+	300	WP_032619177.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ABY64_RS15570	3140025..3140390	+	366	WP_015571639.1	HigA family addiction module antidote protein	Antitoxin
ABY64_RS15575	3140417..3141598	-	1182	WP_032619175.1	PLP-dependent aminotransferase family protein	-
ABY64_RS15580	3141619..3142506	-	888	WP_032619173.1	LysR family transcriptional regulator	-
ABY64_RS15585	3142604..3143206	+	603	WP_017382759.1	short chain dehydrogenase	-
ABY64_RS15590	3143203..3143934	-	732	WP_017382760.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3103261..3140950	37689
	-	flank	IS/Tn	-	-	3135628..3136596	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11564.19 Da
• Isoelectric Point: 9.6776

>T54628 WP_032619177.1 NZ_CP011584:3139715-3140014 [Enterobacter hormaechei]
MINHSRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T54628 NZ_CP011584:3139715-3140014 [Enterobacter hormaechei]
ATGATTAATCATTCTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13705.73 Da
• Isoelectric Point: 9.5936

>AT54628 WP_015571639.1 NZ_CP011584:3140025-3140390 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT54628 NZ_CP011584:3140025-3140390 [Enterobacter hormaechei]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PXC9