Detailed information of TA system TA054600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 476466..477196
• Replicon: chromosome
• Accession: NZ_CP011581
• Organism: Enterobacter hormaechei strain CAV1411

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: ABY65_RS03070
• Protein ID: WP_023302825.1
• Coordinates: 476882..477196 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ABY65_RS03065
• Protein ID: WP_023302826.1
• Coordinates: 476466..476885 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABY65_RS03045	471914..473419	-	1506	WP_017694010.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
ABY65_RS03050	473416..474009	-	594	WP_003861054.1	transcriptional regulator UhpA	-
ABY65_RS03055	474135..474422	-	288	WP_015570113.1	acetolactate synthase small subunit	-
ABY65_RS03060	474426..476114	-	1689	WP_023302827.1	acetolactate synthase large subunit	-
ABY65_RS25190	476221..476319	-	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
ABY65_RS03065	476466..476885	-	420	WP_023302826.1	helix-turn-helix domain-containing protein	Antitoxin
ABY65_RS03070	476882..477196	-	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
ABY65_RS24200	477683..477772	+	90	WP_017383683.1	type I toxin-antitoxin system toxin TisB	-
ABY65_RS03080	477837..478283	+	447	WP_003861064.1	GNAT family N-acetyltransferase	-
ABY65_RS03085	478346..479179	+	834	WP_032620535.1	DMT family transporter	-
ABY65_RS03090	479355..480539	+	1185	WP_015570118.1	multidrug efflux MFS transporter EmrD	-
ABY65_RS03095	480978..482177	+	1200	WP_014603550.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	469639..520672	51033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T54600 WP_023302825.1 NZ_CP011581:c477196-476882 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T54600 NZ_CP011581:c477196-476882 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15380.96 Da
• Isoelectric Point: 5.2068

>AT54600 WP_023302826.1 NZ_CP011581:c476885-476466 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANGPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT54600 NZ_CP011581:c476885-476466 [Enterobacter hormaechei]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGTCCTTTGCTGGATATTGTGT
GCGCCAGAATTAGCCATTACGAAGCTAATGGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTAAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACCCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CTGCGTTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A837FIL2