Detailed information of TA system TA054554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3677253..3677928
• Replicon: chromosome
• Accession: NZ_CP011572
• Organism: Enterobacter hormaechei strain CAV1311

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ABY62_RS18380
• Protein ID: WP_032619177.1
• Coordinates: 3677629..3677928 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0PXC9
• Locus tag: ABY62_RS18375
• Protein ID: WP_015571639.1
• Coordinates: 3677253..3677618 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABY62_RS18355	3673709..3674440	+	732	WP_017382760.1	methyltransferase domain-containing protein	-
ABY62_RS18360	3674437..3675039	-	603	WP_017382759.1	short chain dehydrogenase	-
ABY62_RS18365	3675137..3676024	+	888	WP_032619173.1	LysR family transcriptional regulator	-
ABY62_RS18370	3676045..3677226	+	1182	WP_032619175.1	PLP-dependent aminotransferase family protein	-
ABY62_RS18375	3677253..3677618	-	366	WP_015571639.1	HigA family addiction module antidote protein	Antitoxin
ABY62_RS18380	3677629..3677928	-	300	WP_032619177.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ABY62_RS18385	3678179..3679339	-	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
ABY62_RS18390	3679359..3681014	-	1656	Protein_3454	ATP-binding cassette domain-containing protein	-
ABY62_RS18395	3681047..3682015	+	969	WP_080288382.1	IS5-like element IS903B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3676693..3714384	37691
	-	flank	IS/Tn	-	-	3681092..3682015	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11564.19 Da
• Isoelectric Point: 9.6776

>T54554 WP_032619177.1 NZ_CP011572:c3677928-3677629 [Enterobacter hormaechei]
MINHSRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T54554 NZ_CP011572:c3677928-3677629 [Enterobacter hormaechei]
ATGATTAATCATTCTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13705.73 Da
• Isoelectric Point: 9.5936

>AT54554 WP_015571639.1 NZ_CP011572:c3677618-3677253 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT54554 NZ_CP011572:c3677618-3677253 [Enterobacter hormaechei]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PXC9