Detailed information of TA system TA054543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3373212..3373787
• Replicon: chromosome
• Accession: NZ_CP011568
• Organism: Pandoraea thiooxydans strain DSM 25325

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0G3ENY8
• Locus tag: ABW99_RS15575
• Protein ID: WP_047214677.1
• Coordinates: 3373509..3373787 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: ABW99_RS15570
• Protein ID: WP_047214676.1
• Coordinates: 3373212..3373499 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABW99_RS15550	3368695..3369723	-	1029	WP_083566776.1	porin	-
ABW99_RS15555	3369904..3371241	-	1338	WP_047214674.1	MFS transporter	-
ABW99_RS15560	3371371..3372363	+	993	WP_083566777.1	LysR family transcriptional regulator	-
ABW99_RS15565	3372673..3373005	+	333	WP_047214675.1	type II toxin-antitoxin system HicB family antitoxin	-
ABW99_RS15570	3373212..3373499	-	288	WP_047214676.1	HigA family addiction module antidote protein	Antitoxin
ABW99_RS15575	3373509..3373787	-	279	WP_047214677.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ABW99_RS15580	3374239..3375291	+	1053	WP_083566884.1	alcohol dehydrogenase family protein	-
ABW99_RS15585	3375435..3376007	+	573	WP_047214679.1	NAD(P)H-dependent oxidoreductase	-
ABW99_RS21130	3376332..3376850	+	519	WP_206093650.1	hypothetical protein	-
ABW99_RS15590	3376878..3378113	+	1236	WP_206093651.1	ATP-binding cassette domain-containing protein	-
ABW99_RS15595	3378086..3378658	-	573	WP_047214680.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10406.76 Da
• Isoelectric Point: 10.0370

>T54543 WP_047214677.1 NZ_CP011568:c3373787-3373509 [Pandoraea thiooxydans]
MIKTFRHKGLEAFYRTGNKGGIQPRHAGKLRLLLTALDSAAGPQDMNAPAWRLHSLTGDLAGHWSIWVNGNWRVTFRFNG
ADAELVDYQDYH

Nucleotide

• Length: 279 bp

>T54543 NZ_CP011568:c3373787-3373509 [Pandoraea thiooxydans]
ATGATAAAGACCTTTCGCCATAAGGGGTTAGAAGCTTTTTATCGCACAGGTAACAAAGGGGGCATCCAGCCACGCCATGC
CGGAAAGTTGCGCTTGTTGCTGACCGCTCTGGACAGTGCCGCTGGTCCACAGGACATGAATGCGCCGGCGTGGCGCCTGC
ACTCGTTGACTGGTGATTTGGCTGGTCACTGGTCGATCTGGGTGAACGGCAATTGGCGCGTGACGTTCCGCTTCAACGGC
GCAGATGCCGAACTGGTCGATTATCAGGATTACCACTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10712.58 Da
• Isoelectric Point: 11.1366

>AT54543 WP_047214676.1 NZ_CP011568:c3373499-3373212 [Pandoraea thiooxydans]
MSKMHNPAHPGEVLRQWLPQDMTVTEAAQALQVSRITLSKVLNGKAGVTAGMALRLSAWLGTSPELWLGMQNQWDLWQAE
QQPKPKIKPLKRMRV

Nucleotide

• Length: 288 bp

>AT54543 NZ_CP011568:c3373499-3373212 [Pandoraea thiooxydans]
ATGAGCAAGATGCACAACCCCGCTCACCCCGGCGAAGTGTTGCGGCAATGGTTGCCGCAGGACATGACGGTCACGGAAGC
GGCCCAGGCGTTACAGGTCTCCCGCATTACGCTGTCCAAGGTGTTGAACGGCAAGGCAGGCGTGACTGCGGGCATGGCGT
TGCGCCTTTCGGCATGGCTGGGCACGTCGCCCGAGTTGTGGCTGGGGATGCAAAATCAGTGGGATCTGTGGCAGGCGGAG
CAGCAGCCAAAGCCGAAGATAAAGCCGCTCAAACGCATGCGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0G3ENY8