Detailed information of TA system TA054528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 914435..914927
• Replicon: chromosome
• Accession: NZ_CP011545
• Organism: Corynebacterium testudinoris strain DSM 44614

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: CTEST_RS04505
• Protein ID: WP_047252729.1
• Coordinates: 914435..914689 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: CTEST_RS04510
• Protein ID: WP_047252730.1
• Coordinates: 914670..914927 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTEST_RS13720	910130..910909	+	780	WP_162488752.1	CPBP family intramembrane metalloprotease	-
CTEST_RS13025	911243..911842	+	600	WP_144413219.1	hypothetical protein	-
CTEST_RS13030	912018..912590	+	573	WP_052844299.1	hypothetical protein	-
CTEST_RS04495	912823..913950	+	1128	WP_047252727.1	low temperature requirement protein A	-
CTEST_RS04500	913988..914428	+	441	WP_047252728.1	YtoQ family protein	-
CTEST_RS04505	914435..914689	-	255	WP_047252729.1	Txe/YoeB family addiction module toxin	Toxin
CTEST_RS04510	914670..914927	-	258	WP_047252730.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CTEST_RS04515	914937..915629	-	693	WP_047252731.1	type 1 glutamine amidotransferase domain-containing protein	-
CTEST_RS04520	915640..916206	-	567	WP_047252732.1	TetR/AcrR family transcriptional regulator	-
CTEST_RS04525	916309..917586	-	1278	WP_047252733.1	NADH:flavin oxidoreductase/NADH oxidase family protein	-
CTEST_RS04530	917619..918674	-	1056	WP_047252734.1	hypothetical protein	-
CTEST_RS04535	918737..919222	+	486	WP_047252735.1	DinB family protein	-
CTEST_RS04540	919207..919587	-	381	WP_144413220.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	908698..910110	1412

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10179.62 Da
• Isoelectric Point: 8.8754

>T54528 WP_047252729.1 NZ_CP011545:c914689-914435 [Corynebacterium testudinoris]
MPPVWDEHAWDDYLWWQTQDRKVLKRINLLVKDIVRNGNEGIGKPEPLKHDFAGYWSRRVTDEHRLVYKLTATELRIAAC
RYHY

Nucleotide

• Length: 255 bp

>T54528 NZ_CP011545:c914689-914435 [Corynebacterium testudinoris]
ATGCCGCCTGTCTGGGATGAGCATGCGTGGGACGACTATCTCTGGTGGCAAACGCAAGACCGAAAAGTACTGAAAAGAAT
CAACCTGCTAGTGAAAGACATTGTCCGAAACGGCAATGAGGGGATTGGCAAACCGGAGCCGCTGAAGCACGACTTCGCCG
GCTACTGGTCTCGCCGCGTCACCGACGAGCACCGGCTCGTCTATAAACTCACCGCAACAGAGCTTCGCATTGCCGCCTGC
CGCTATCACTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.60 Da
• Isoelectric Point: 4.7914

>AT54528 WP_047252730.1 NZ_CP011545:c914927-914670 [Corynebacterium testudinoris]
MRTMSYTESRANYAQVLDDVTNDREAIVVTRAGHDPVVMVSLADYESLRETAYLLRSPANARRLLDAMERLEAGRGEPHD
AACLG

Nucleotide

• Length: 258 bp

>AT54528 NZ_CP011545:c914927-914670 [Corynebacterium testudinoris]
ATGAGAACTATGAGCTACACCGAGTCACGTGCCAACTACGCACAGGTTCTGGACGATGTGACCAATGATCGCGAAGCAAT
CGTCGTTACTCGAGCCGGACACGACCCTGTCGTCATGGTCTCGCTCGCCGATTACGAATCCCTTCGAGAAACTGCTTACC
TGCTCCGCTCCCCCGCTAACGCGCGGCGTCTTCTCGACGCCATGGAACGCCTTGAAGCCGGCCGCGGCGAACCACATGAT
GCCGCCTGTCTGGGATGA