Detailed information of TA system TA054527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1187596..1188185
• Replicon: chromosome
• Accession: NZ_CP011542
• Organism: Corynebacterium mustelae strain DSM 45274

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A0G3GWD1
• Locus tag: CMUST_RS05570
• Protein ID: WP_047261679.1
• Coordinates: 1187596..1187877 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CMUST_RS05575
• Protein ID: WP_047261680.1
• Coordinates: 1187886..1188185 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CMUST_RS05550	1182695..1184188	-	1494	WP_144414132.1	AI-2E family transporter	-
CMUST_RS05555	1184388..1184585	-	198	WP_047261676.1	methionine/alanine import NSS transporter subunit MetS	-
CMUST_RS05560	1184582..1186225	-	1644	WP_047261677.1	sodium-dependent transporter	-
CMUST_RS05565	1186470..1187555	+	1086	WP_047261678.1	redox-regulated ATPase YchF	-
CMUST_RS05570	1187596..1187877	+	282	WP_047261679.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CMUST_RS05575	1187886..1188185	+	300	WP_047261680.1	HigA family addiction module antidote protein	Antitoxin
CMUST_RS05580	1189078..1189566	+	489	WP_047261681.1	hypothetical protein	-
CMUST_RS16300	1189723..1189941	-	219	Protein_1097	transposase	-
CMUST_RS16305	1189919..1190143	-	225	WP_083987427.1	hypothetical protein	-
CMUST_RS16530	1190088..1190249	-	162	WP_158408200.1	DDE-type integrase/transposase/recombinase	-
CMUST_RS16535	1190311..1190412	-	102	WP_144414134.1	hypothetical protein	-
CMUST_RS16885	1190638..1190892	-	255	WP_162488701.1	hypothetical protein	-
CMUST_RS16315	1191082..1191165	+	84	WP_083987705.1	PLDc N-terminal domain-containing protein	-
CMUST_RS05595	1191347..1191778	+	432	WP_047261682.1	hypothetical protein	-
CMUST_RS05600	1192162..1192995	+	834	WP_047261683.1	hydroxyethylthiazole kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-82)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10849.31 Da
• Isoelectric Point: 10.7810

>T54527 WP_047261679.1 NZ_CP011542:1187596-1187877 [Corynebacterium mustelae]
VIISFGDRDTERVWRREAVTKLDPRIHKTANRKLHQLDAATTLDTLRVPPGNRLEALKGNRKGRYSIRVNKQWRITFRWT
DGGPEDVTVEDCH

Nucleotide

• Length: 282 bp

>T54527 NZ_CP011542:1187596-1187877 [Corynebacterium mustelae]
GTGATCATATCTTTTGGTGACCGGGACACCGAACGGGTATGGCGGCGTGAAGCTGTGACAAAGCTTGATCCACGGATTCA
TAAAACTGCTAACCGGAAACTTCATCAACTTGATGCAGCCACGACCCTTGACACACTGAGGGTGCCGCCAGGTAATCGCT
TGGAGGCGCTCAAAGGCAACCGAAAGGGTCGCTACAGTATCCGGGTGAACAAACAGTGGCGGATTACATTTCGGTGGACT
GATGGTGGTCCCGAAGACGTAACTGTCGAAGATTGTCATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11104.82 Da
• Isoelectric Point: 5.1489

>AT54527 WP_047261680.1 NZ_CP011542:1187886-1188185 [Corynebacterium mustelae]
MTEKLYPPIHPGEVLMEDFIEGFGITQNKLAVSIGVPPRRINEIVHGKRGITADTALRLGKYFGIEPKFWLNLQSRYELE
LAEEKVGEQISAIQPLDAA

Nucleotide

• Length: 300 bp

>AT54527 NZ_CP011542:1187886-1188185 [Corynebacterium mustelae]
ATGACTGAGAAGCTTTATCCTCCGATTCACCCTGGTGAAGTCTTGATGGAGGATTTCATTGAGGGATTCGGGATAACCCA
GAACAAACTCGCTGTGTCAATTGGCGTGCCACCGCGCCGAATCAACGAGATCGTGCACGGGAAGCGGGGAATCACTGCGG
ACACCGCACTTCGGCTGGGGAAGTACTTTGGTATAGAGCCAAAGTTTTGGTTAAACCTGCAATCACGATACGAGCTCGAA
CTGGCAGAGGAAAAGGTCGGCGAGCAAATCTCGGCGATTCAACCCCTCGACGCCGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0G3GWD1